Delta4 - desaturase genes and uses thereof
First Claim
Patent Images
1. An isolated nucleotide sequence corresponding to or complementary to at least about 50% of the nucleotide sequence selected from the group consisting of SEQ ID NO:
- 14, SEQ ID NO;
15, SEQ ID NO;
16 and SEQ ID NO;
17.
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Abstract
The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 4 (i.e., “Δ4-desaturase”). In particular, Δ4-desaturase may be utilized, for example, in the conversion of adrenic acid to ω6-docosapentaenoic acid and in the conversion of ω3-docosapentaenoic acid to docosahexaenoic acid. The polyunsaturated fatty acids produced by use of the enzyme may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
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Citations
49 Claims
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1. An isolated nucleotide sequence corresponding to or complementary to at least about 50% of the nucleotide sequence selected from the group consisting of SEQ ID NO:
- 14, SEQ ID NO;
15, SEQ ID NO;
16 and SEQ ID NO;
17. - View Dependent Claims (2, 3, 4, 5, 6, 43, 44, 45)
- 14, SEQ ID NO;
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7. A purified polypeptide which desaturates polyunsaturated fatty acids at carbon 4 and has at least about 50% amino acid similarity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 18, SEQ ID NO;
19, SEQ ID NO;
20 and SEQ ID NO;
21.
- 18, SEQ ID NO;
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8. A method of producing a desaturase comprising the steps of:
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a) isolating said nucleotide sequence represented selected from the group consisting of SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16 and SEQ ID NO;
17;
b) constructing a vector comprising;
i) said isolated nucleotide sequence operably linked to ii) a promoter;
c) introducing said vector into a host cell under time and conditions sufficient for expression of said desaturase. - View Dependent Claims (9, 10, 11, 12, 13)
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14. A vector comprising:
- a) a nucleotide sequence selected from the group consisting of SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16 and SEQ ID NO;
17, operably linked to b) a promoter. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
- a) a nucleotide sequence selected from the group consisting of SEQ ID NO;
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25. A transgenic, non-human mammal whose genome comprises a nucleotide sequence encoding Δ
- 4-desaturase, operably linked to a promoter.
- View Dependent Claims (26, 27)
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28. A method for producing a polyunsaturated fatty acid comprising the steps of:
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a) isolating a nucleotide sequence selected from the group consisting of SEQ ID NO;
14, SEQ ID NO;
15, SEQ ID NO;
16 and SEQ ID NO;
17;
b) constructing a vector comprising said isolated nucleotide sequence;
c) introducing said vector into a host cell under time and conditions sufficient for expression of Δ
4-desaturase; and
d) exposing said expressed Δ
4-desaturase to a substrate polyunsaturated fatty acid in order to convert said substrate to a product polyunsaturated fatty acid. - View Dependent Claims (29, 30, 31, 35, 36, 37, 38, 39, 40, 41, 42, 46, 47, 48)
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32. A method of producing a polyunsaturated fatty acid comprising the steps of:
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a) exposing a substrate polyunsaturated fatty acid to one or more enzymes selected from the group consisting of a desaturase and an elongase in order to convert said substrate to a product polyunsaturated fatty acid; and
b) exposing said product polyunsaturated fatty acid of step (a) to a Δ
4-desaturase comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
18, SEQ ID NO;
19, SEQ ID NO;
20 and SEQ ID NO;
21, in order to convert said product polyunsaturated fatty acid to a final product polyunsaturated fatty acid. - View Dependent Claims (33, 34, 49)
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Specification