Maturation of antigen-presenting cells using activated T cells
First Claim
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1. A method for maturing dendritic cells, comprising:
- (a) providing a population of cells wherein at least a portion thereof comprises immature dendritic cells; and
(b) exposing the population of cells to activated T cells or supernatant therefrom, thereby inducing maturation.
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Abstract
The present invention relates to methods for maturing antigen-presenting cells, and more particularly, to methods for maturing dendritic cells. Methods for generating mature and/or maturing antigen-presenting cells in vitro and in vivo are disclosed. The present invention also relates to compositions of cells, including mature antigen-presenting cells and/or activated T cells and their use in generating immune responses in vivo, and inhibiting the development of or preventing infectious diseases and cancers.
83 Citations
81 Claims
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1. A method for maturing dendritic cells, comprising:
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(a) providing a population of cells wherein at least a portion thereof comprises immature dendritic cells; and
(b) exposing the population of cells to activated T cells or supernatant therefrom, thereby inducing maturation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53)
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- 54. A composition comprising dendritic cells and activated T cells wherein the dendritic cells have been matured by exposure to activated T cells or supernatant therefrom ex vivo.
- 61. A method for reducing the presence of cancer cells in a mammal, comprising administering to the mammal a composition comprising, dendritic cells matured by activated T cells or supernatant therefrom ex vivo, activated T cells, and a pharmaceutically acceptable excipient.
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64. A method for generating mature dendritic cells in vivo comprising, administering to a mammal a composition comprising activated T cells.
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65. A method for generating mature dendritic cells, comprising:
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(a) generating immature dendritic cells in vitro from a source of precursor cells by a method selected from the group consisting of;
i. exposing the precursor cells to GM-CSF and IL-4;
ii. exposing the precursor cells to GM-CSF and IL-13;
iii. exposing the precursor cells to activated T cells or supernatant therefrom;
iv. exposing the precursor cells to GM-CSF and IL-4 and activated T cells or supernatant therefrom; and
v. exposing the precursor cells to GM-CSF and IL-13 and activated T cells or supernatant therefrom;
(b) administering to a mammal the immature dendritic cells of part (a), and;
(c) administering to the mammal activated T cells, thereby inducing in vivo maturation of the immature dendritic cells. - View Dependent Claims (66, 67, 68, 69, 70, 71, 72, 73, 75, 76, 77, 78, 79, 80)
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74. A method for generating mature dendritic cells, comprising:
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(a) obtaining a population of cells from a mammal wherein at least a portion thereof comprises precursor dendritic cells;
(b) exposing said portion of cells in vitro to GM-CSF and IL-4 or IL-13 to generate immature dendritic cells; and
(c) exposing said immature dendritic cells in vitro to a population of activated T cells or supernatant therefrom for a sufficient period of time to achieve desired maturation.
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81. A method for expanding dendritic/tumor cell fusions comprising exposing the dendritic/tumor cell fusions to activated T cells or supernatant therefrom.
Specification