Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acid levels
First Claim
Patent Images
1. An isolated polynucleotide selected from the group consisting of:
- a) a polynucleotide comprising SEQ ID NO;
1;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
1 over the entire length of SEQ ID NO;
1;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
1 over the entire length of SEQ ID NO;
1;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
1 over the entire length of SEQ ID NO;
1;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
1 over the entire length of SEQ ID NO;
1;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
1 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
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Abstract
By this invention, novel nucleic acid sequences are provided, wherein said nucleic acid sequence is a genomic sequence of a plant desaturase encoding sequence. Also provided in the present invention are the promoter and intron sequences of the desaturase genomic sequences. Furthermore, recombinant DNA constructs employing the polynucleotide sequences are provided. The instant invention also provides methods for the modification of fatty acid compositions in host plant cells.
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Citations
48 Claims
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1. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
1;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
1 over the entire length of SEQ ID NO;
1;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
1 over the entire length of SEQ ID NO;
1;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
1 over the entire length of SEQ ID NO;
1;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
1 over the entire length of SEQ ID NO;
1;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
1 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f). - View Dependent Claims (20, 21, 22, 25, 26, 27, 28, 29)
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2. An isolated polynucleotide selected from the group consisting of:
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a) a polynucleotide comprising SEQ ID NO;
2;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
2 over the entire length of SEQ ID NO;
2;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
2 over the entire length of SEQ ID NO;
2;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
2 over the entire length of SEQ ID NO;
2;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
2 over the entire length of SEQ ID NO;
2;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
2 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
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3. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
3;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
3 over the entire length of SEQ ID NO;
3;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
3 over the entire length of SEQ ID NO;
3;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
3 over the entire length of SEQ ID NO;
3;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
3 over the entire length of SEQ ID NO;
3;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
3 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
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4. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
4;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
4 over the entire length of SEQ ID NO;
4;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
4 over the entire length of SEQ ID NO;
4;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
4 over the entire length of SEQ ID NO;
4;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
4 over the entire length of SEQ ID NO;
4;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
4 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
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5. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
5;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
5 over the entire length of SEQ ID NO;
5;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
5 over the entire length of SEQ ID NO;
5;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
5 over the entire length of SEQ ID NO;
5;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
5 over the entire length of SEQ ID NO;
5;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
5 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
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6. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
6;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
6 over the entire length of SEQ ID NO;
6;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
6 over the entire length of SEQ ID NO;
6;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
6 over the entire length of SEQ ID NO;
6;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
6 over the entire length of SEQ ID NO;
6;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
6 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
-
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7. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
7;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
7 over the entire length of SEQ ID NO;
7;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
7 over the entire length of SEQ ID NO;
7;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
7 over the entire length of SEQ ID NO;
7;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
7 over the entire length of SEQ ID NO;
7;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
7 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
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8. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
8;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
8 over the entire length of SEQ ID NO;
8;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
8 over the entire length of SEQ ID NO;
8;
d) a polynucleotide sequence having at least 90% identity to that of SEQ i) NO;
8 over the entire length of SEQ ID NO;
8;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
8 over the entire length of SEQ i) NO;
8;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
8 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
-
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9. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
23;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
23 over the entire length of SEQ ID NO;
23;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
23 over the entire length of SEQ ID NO;
23;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
23 over the entire length of SEQ ID NO;
23;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
23 over the entire length of SEQ ID NO;
23;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
23 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
-
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10. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
24;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
24 over the entire length of SEQ ID NO;
24;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
24 over the entire length of SEQ ID NO;
24;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
24 over the entire length of SEQ ID NO;
24;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
24 over the entire length of SEQ ID NO;
24;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
24 or a fragment thereof; and
g) a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
-
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11. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
25;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
25 over the entire length of SEQ ID NO;
25;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
25 over the entire length of SEQ ID NO;
25;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
25 over the entire length of SEQ ID NO;
25;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
25 over the entire length of SEQ ID NO;
25;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
25 or a fragment thereof; and
a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
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12. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
26;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
26 over the entire length of SEQ ID NO;
26;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
26 over the entire length of SEQ ID NO;
26;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
26 over the entire length of SEQ ID NO;
26;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
26 over the entire length of SEQ ID NO;
26;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
26 or a fragment thereof; and
a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
-
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13. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
27;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
27 over the entire length of SEQ ID NO;
27;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
27 over the entire length of SEQ ID NO;
27;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
27 over the entire length of SEQ ID NO;
27;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
27 over the entire length of SEQ ID NO;
27;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
27 or a fragment thereof; and
a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
-
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14. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
28;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
28 over the entire length of SEQ ID NO;
28;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
28 over the entire length of SEQ ID NO;
28;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
28 over the entire length of SEQ ID NO;
28;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
28 over the entire length of SEQ ID NO;
28;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
28 or a fragment thereof; and
a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
-
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15. An isolated polynucleotide selected from the group consisting of:
-
a) a polynucleotide comprising SEQ ID NO;
29;
b) a polynucleotide sequence having at least 70% identity to that of SEQ ID NO;
29 over the entire length of SEQ ID NO;
29;
c) a polynucleotide sequence having at least 80% identity to that of SEQ ID NO;
29 over the entire length of SEQ ID NO;
29;
d) a polynucleotide sequence having at least 90% identity to that of SEQ ID NO;
29 over the entire length of SEQ ID NO;
29;
e) a polynucleotide sequence having at least 95% identity to that of SEQ ID NO;
29 over the entire length of SEQ ID NO;
29;
f) an polynucleotide sequence that hybridizes, under stringent conditions, to SEQ ID NO;
29 or a fragment thereof; and
a polynucleotide sequence complementary to the polynucleotide sequence of (a), (b), (c), (d), (e), or (f).
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16. An intron obtained from a genomic polynucleotide sequence selected from the group consisting of:
-
a) a genomic polynucleotide sequence having at least 70% identity to coding regions of SEQ ID NO;
1 over the entire coding regions of SEQ ID NO;
1;
b) a genomic polynucleotide sequence having at least 80% identity to coding regions of SEQ ID NO;
1 over the entire coding regions of SEQ ID NO;
1;
c) a genomic polynucleotide sequence having at least 90% identity to coding regions of SEQ ID NO;
1 over the entire coding regions of SEQ ID NO;
1; and
d) a genomic polynucleotide sequence having at least 95% identity to coding regions of SEQ ID NO;
1 over the entire coding regions of SEQ ID NO;
1.
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17. An intron obtained from a genomic polynucleotide sequence selected from the group consisting of:
-
a) a genomic polynucleotide sequence having at least 70% identity to coding regions of SEQ ID NO;
3 over the entire coding regions of SEQ ID NO;
3;
b) a genomic polynucleotide sequence having at least 80% identity to coding regions of SEQ ID NO;
3 over the entire coding regions of SEQ ID NO;
3;
c) a genomic polynucleotide sequence having at least 90% identity to coding regions of SEQ ID NO;
3 over the entire coding regions of SEQ ID NO;
3; and
d) a genomic polynucleotide sequence having at least 95% identity to coding regions of SEQ ID NO;
3 over the entire coding regions of SEQ ID NO;
3.
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18. An intron obtained from a genomic polynucleotide sequence selected from the group consisting of:
-
a) a genomic polynucleotide sequence having at least 70% identity to coding regions of SEQ ID NO;
23 over the entire coding regions of SEQ ID NO;
23;
b) a genomic polynucleotide sequence having at least 80% identity to coding regions of SEQ ID NO;
23 over the entire coding regions of SEQ ID NO;
23;
c) a genomic polynucleotide sequence having at least 90% identity to coding regions of SEQ ID NO;
23 over the entire coding regions of SEQ ID NO;
23; and
d) a genomic polynucleotide sequence having at least 95% identity to coding regions of SEQ ID NO;
23 over the entire coding regions of SEQ ID NO;
23.
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19. An intron obtained from a genomic polynucleotide sequence selected from the group consisting of:
-
a) a genomic polynucleotide sequence having at least 70% identity to coding regions of SEQ ID NO;
27 over the entire coding regions of SEQ ID NO;
27;
b) a genomic polynucleotide sequence having at least 80% identity to coding regions of SEQ ID NO;
27 over the entire coding regions of SEQ ID NO;
27;
c) a genomic polynucleotide sequence having at least 90% identity to coding regions of SEQ ID NO;
27 over the entire coding regions of SEQ ID NO;
27; and
d) a genomic polynucleotide sequence having at least 95% identity to coding regions of SEQ ID NO;
27 over the entire coding regions of SEQ ID NO;
27.
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23. A plant cell comprising the DNA construct of 20.
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24. A plant comprising the cell of claim 24.
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30. A method of inhibiting gene expression in a plant cell, comprising the steps of:
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transforming a plant cell with a DNA construct comprising a non-coding region of a gene to be inhibited positioned in a sense orientation; and
growing said cell under conditions wherein transcription of said non-coding region is initiated, whereby said expression of said gene is inhibited. - View Dependent Claims (31, 32, 33)
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34. A method of inhibiting desaturase expression in a plant cell, comprising the steps of:
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transforming a plant cell with a DNA construct comprising a non-coding region of a gene encoding desaturase positioned in an antisense orientation; and
growing said cell under conditions wherein transcription of said non-coding region is initiated, whereby said expression of said desaturase is inhibited. - View Dependent Claims (35, 36, 37, 38, 39)
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40. A method of modifying fatty acid composition in a plant cell, comprising the steps of:
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transforming a plant cell with a DNA construct comprising a non-coding region of a gene encoding desaturase positioned in a sense or an antisense orientation; and
growing said cell under conditions wherein transcription of said non-coding region is initiated, whereby said expression of said desaturase is inhibited. - View Dependent Claims (41, 42, 43, 44, 45)
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46. A method of inhibiting desaturase expression in a plant cell, comprising the steps of:
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transforming a plant cell with a DNA construct comprising a nucleic acid sequence capable of binding to or cleaving a non-coding region of a gene encoding desaturase; and
growing said cell under conditions wherein transcription of said nucleic acid sequence is initiated, whereby said expression of said desaturase is inhibited. - View Dependent Claims (47)
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48. A oil seed composition comprising:
at least about 50-75% oleic acid, no more than about 10-30% linoleic acid and no more than about 1-3% linolenic acid.
Specification