Means and methods for detection of binding of members of specific binding pairs
First Claim
1. A method for detecting binding of members of a specific binding pair comprising providing a first member of said binding pair coupled to a deposition nucleus and specifically binding said first member to a surface-immobilized second member of said pair and determining the electrical resistance of said surface, the method characterized in that after binding of the members on said surface an electrically conductive deposit is formed on said surface under conditions that allow said deposit to be formed specifically on said nucleus or deposit formed.
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Abstract
The present invention relates to an affinity sensor and methods suitable for use in an affinity sensor for detecting specific molecular binding events, as is particularly used in the molecular biological field, for example, in the medical diagnostics, in the biosensor technology or in the DNA-microarray technology, and application of the same. A method for detecting binding of members of a specific binding pair of the invention comprises providing a first member of said binding pair coupled to a deposition nucleus and specifically binding said first member to a surface-immobilized second member of said pair and determining the electrical resistance of said surface, the method characterized in that after binding of the members on said surface an electrically conductive deposit is formed on said surface under conditions that allow said deposit to be formed specifically on said nucleus or deposit formed.
31 Citations
21 Claims
- 1. A method for detecting binding of members of a specific binding pair comprising providing a first member of said binding pair coupled to a deposition nucleus and specifically binding said first member to a surface-immobilized second member of said pair and determining the electrical resistance of said surface, the method characterized in that after binding of the members on said surface an electrically conductive deposit is formed on said surface under conditions that allow said deposit to be formed specifically on said nucleus or deposit formed.
- 8. A sensor comprising electrodes in contact with a surface comprising a binding pair wherein a member of said binding pair comprises a deposition nucleus and wherein said deposition nucleus comprises an electrically conductive deposit.
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14. A method for quantifying a member of a specific binding pair in a sample comprising
linking the member in said sample with a deposition nucleus, contacting a surface comprising an immobilized second member of said pair with said sample under conditions allowing specific binding of said pair, subsequently performing an enhancement step on said surface by depositing an electrically conductive deposit on said nucleus and/or deposit formed, and determining the electrical resistance of said surface and optionally comparing the electrical resistance of said surface with a reference.
Specification