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Microfluidic sequencing methods

  • US 20030087300A1
  • Filed: 11/22/2002
  • Published: 05/08/2003
  • Est. Priority Date: 04/04/1997
  • Status: Active Grant
First Claim
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1. A method of sequencing a nucleic acid comprising:

  • providing a target nucleic acid, a first sequencing primer, a polymerase, dNTPs, and ddNTPs;

    mixing the target nucleic acid, the first sequencing primer, the polymerase, the dNTPs, and the ddNTPs in a microfluidic device under conditions permitting target dependent polymerization of the dNTPs, thereby providing polymerization products; and

    , separating the polymerization products by size in the microfluidic device to provide a sequence of the target nucleic acid.

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