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Biological method for the production of adipic acid and intermediates

  • US 20030087403A1
  • Filed: 10/16/2002
  • Published: 05/08/2003
  • Est. Priority Date: 02/19/1999
  • Status: Active Grant
First Claim
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1. An isolated nucleic acid fragment encoding an adipic acid synthesizing enzyme selected from the group consisting of:

  • (a) an isolated nucleic acid molecule encoding the amino acid sequence set forth in SEQ ID NO;

    12, SEQ ID NO;

    20, SEQ ID NO;

    24, and SEQ ID NO;

    26, or an enzymatically active fragment thereof;

    (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions;

    0.1×

    SSC, 0.1% SDS at 65°

    C.; and

    washed with 2×

    SSC, 0.1% SDS followed by 0.1×

    SSC, 0.1% SDS; and

    (c) an isolated nucleic acid molecule that is completely complementary to (a) or (b).

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