Fluorescent dye
First Claim
1. A fluorescent cyanine dye having the formula:
- T1(—
CH═
)n1A (—
CH═
)n2T2wherein;
n≧
1 and n1 is the same as or different from n2;
A comprises the formula;
wherein;
X1 and Y1 are selected from the group consisting of C(CH3)2, CH═
CH, O, N, S, Se and Te and either X1 or Y1 is N;
X2 and Y2 are selected from the group consisting of C(CH3)2, CH═
CH, O, N, S, Se and Te and either X2 or Y2 is N;
or A comprises the formula;
wherein;
Z1 and Y1 are selected from the group consisting of C(CH3)2, CH═
CH, O, N, S, Se and Te and either Z1 or Y1 is N;
Z2 and Y2 are selected from the group consisting of C(CH3)2, CH═
CH, O, N, S, Se and Te and either Z2 or Y2 is N; and
wherein a and b are 0 or 1, and a+b=1; and
where X, Y or Z is N, R2 and R3 are substituents on N and are the same or different and are selected from the group consisting of H, methyl, ethyl, C(CH3)2 and (CH2)qV, wherein q is an integer from 1 to 25 and V is a reactive group or H; and
wherein;
T1 and T2 are the same or different and have the formula;
wherein;
Q is selected from the group consisting of O, S, CH2, (CH═
CH) and C(CH3)2;
R1 and R4 are the same or different and are selected from the group consisting of H, methyl, ethyl and (CH2)qV, wherein q is an integer from 1 to 25 and V is a reactive group or H;
each of W1-8 is the same or different and may be H or a hydrophilic moiety;
at least one occurrence of W is a hydrophilic moiety; and
wherein at least one of R1-R4 has a reactive group.
3 Assignments
0 Petitions
Accused Products
Abstract
The invention relates to fluorescent dyes. More particularly, the invention relates to fluorescent cyanine dyes, and especially to water soluble fluorescent cyanine dyes that contain additional sites for attachment to biomolecules. The invention provides a group of novel, water soluble fluorescent cyanine dyes that have distinct fluorescence characteristics that permit their use in any assay or method suited to water soluble fluorescent dyes, and especially to assays requiring a plurality of distinguishable fluorescent markers. The invention further relates to nucleotides, nucleosides, polynucleotides and polypeptides labeled with novel fluorescent cyanine dyes according to the invention, and methods of using them.
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Citations
49 Claims
-
1. A fluorescent cyanine dye having the formula:
-
T1(—
CH═
)n1A (—
CH═
)n2T2wherein;
n≧
1 and n1 is the same as or different from n2;
A comprises the formula;
wherein;
X1 and Y1 are selected from the group consisting of C(CH3)2, CH═
CH, O, N, S, Se and Te and either X1 or Y1 is N;
X2 and Y2 are selected from the group consisting of C(CH3)2, CH═
CH, O, N, S, Se and Te and either X2 or Y2 is N;
orA comprises the formula;
wherein;
Z1 and Y1 are selected from the group consisting of C(CH3)2, CH═
CH, O, N, S, Se and Te and either Z1 or Y1 is N;
Z2 and Y2 are selected from the group consisting of C(CH3)2, CH═
CH, O, N, S, Se and Te and either Z2 or Y2 is N; and
wherein a and b are 0 or 1, and a+b=1; and
where X, Y or Z is N, R2 and R3 are substituents on N and are the same or different and are selected from the group consisting of H, methyl, ethyl, C(CH3)2 and (CH2)qV, wherein q is an integer from 1 to 25 and V is a reactive group or H; and
wherein;
T1 and T2 are the same or different and have the formula;
wherein;
Q is selected from the group consisting of O, S, CH2, (CH═
CH) and C(CH3)2;
R1 and R4 are the same or different and are selected from the group consisting of H, methyl, ethyl and (CH2)qV, wherein q is an integer from 1 to 25 and V is a reactive group or H;
each of W1-8 is the same or different and may be H or a hydrophilic moiety;
at least one occurrence of W is a hydrophilic moiety; and
wherein at least one of R1-R4 has a reactive group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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- 13. A fluorescent cyanine dye having the formula:
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15. A fluorescent cyanine dye having the formula:
- 16. A fluorescent cyanine dye having the formula:
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18. A fluorescent cyanine dye having the formula:
- 19. A fluorescent cyanine dye having the formula:
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21. A fluorescent cyanine dye having the formula:
- 22. A fluorescent cyanine dye having the formula:
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24. A fluorescent cyanine dye having the formula:
- 25. A fluorescent cyanine dye having the formula:
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27. A fluorescent cyanine dye having the formula:
- 28. A fluorescent cyanine dye having the formula:
- 30. A fluorescent cyanine dye having the formula:
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31. A fluorescent cyanine dye having the formula:
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33. A fluorescent cyanine dye having the formula:
Specification