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High throughput screening for sequences of interest

  • US 20030092022A1
  • Filed: 05/13/2002
  • Published: 05/15/2003
  • Est. Priority Date: 06/16/1997
  • Status: Abandoned Application
First Claim
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1. A method for identifying a polynucleotide encoding a polypeptide of interest comprising:

  • co-encapsulating in a microenvironment a plurality of library clones containing DNA obtained from a mixed population of organisms, with a mixture of oligonucleotide probes comprising a fluorescence marker and at least a portion of a polynucleotide sequence encoding a polypeptide of interest having a specified bioactivity under such conditions and for such time as to allow interaction of complementary sequences; and

    identifying clones containing a complement to the oligonucleotide probe encoding the polypeptide of interest by separating clones with a fluorescent analyzer that detects fluorescence.

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