Multi-column affinity detection system

• US 20030092056A1
• Filed: 10/31/2002
• Published: 05/15/2003
• Est. Priority Date: 10/31/2001
• Status: Abandoned Application

• Current Assignee: Ebara Corporation
• Original Assignee: Ebara Corporation
• Inventors: Nagasawa, Hiroshi
• Application Number:
  US10/284,442
• Publication Number:
  US 20030092056A1
• Time in Patent Office: Days
• US Class Current: 435/6.14
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G01N 30/466 with separation columns in parallel

Abstract

A multi-column affinity detection system which comprises a plurality of columns having probe molecules fixed thereto that will cause different specific binding reactions and a switching mechanism, said system being capable of simultaneous or sequential detection of many kinds of affinity characteristics. The probe molecules are preferably DNA, RNA or PNA and fragments thereof, oligonucleotides having desired base sequences, antigens, antibodies or epitopes, enzymes, proteins or polypeptide chains at functional sites thereof.

Claims

1. A multi-column affinity detection system which comprises a plurality of columns having probe molecules fixed thereto that will cause different specific binding reactions, a switching mechanism and one or more detectors, said system being capable of simultaneous or sequential detection of many kinds of affinity characteristics with the respective elements being functionally connected together.

2. The multi-column affinity detection system according to claim 1, wherein said columns having probe molecules fixed thereto are monolithic columns or membrane columns.

3. The multi-column affinity detection system according to claim 1 or 2, wherein said probe molecules, are DNA, RNA or PNA and fragments thereof, oligonucleotides having desired base sequences, antigens, antibodies or epitopes, enzymes, proteins or polypeptide chains at functional sites thereof.

4. The multi-column affinity detection system according to any one of claims 1-3, wherein said switching mechanism comprises a continuously operating sampler and a switching valve that is operatively associated with said sampler and which is coupled to said plurality of columns which have probe molecules fixed thereto that will cause different specific binding reactions and wherein identical samples are sequentially introduced in the same quantity into said plurality of columns and the effluents from the respective columns are allowed to converge and then introduced into a single detector, whereby the samples are sequentially loaded into the plurality of different columns for sequential detection.

5. The multi-column affinity detection system according to any one of claims 1-3, wherein said switching mechanism is a splitting sampler capable of simultaneously supplying divided portions of a sample in a constant quantity and wherein branch lines coupled to said sampler are also coupled to said plurality of columns which have probe molecules fixed thereto that will cause different specific binding reactions and wherein identical samples are simultaneously introduced in the same quantity into said plurality of columns and the effluents from the respective columns are introduced into separate detectors, whereby the samples are simultaneously loaded into the plurality of different columns for simultaneous detection.