Apparatus for circulating carrier fluid
First Claim
1. An apparatus for circulating a carrier fluid comprising two or more chambers maintained at different temperatures, each chamber comprising:
- an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber (inlet pneumatic air pressure port); and
an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber (outlet pneumatic air pressure port);
wherein the chambers are sequentially connected such that the outlet valve of one chamber is connected to the inlet valve of an adjacent chamber in a direction the fluid flows.
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Accused Products
Abstract
Provided are an apparatus for circulating a carrier fluid having two or more chambers or sections, an apparatus for amplifying a nucleic acid using the same, and a chip containing the same. The apparatus for circulating a carrier fluid includes two or more chambers maintained at different temperatures, each chamber having an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber (inlet pneumatic air pressure port), and an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber (outlet pneumatic air pressure port), wherein the chambers are sequentially connected such that the outlet valve of one chamber is connected to the inlet valve of an adjacent chamber in a direction the fluid flows.
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Citations
17 Claims
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1. An apparatus for circulating a carrier fluid comprising two or more chambers maintained at different temperatures, each chamber comprising:
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an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber (inlet pneumatic air pressure port); and
an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber (outlet pneumatic air pressure port);
wherein the chambers are sequentially connected such that the outlet valve of one chamber is connected to the inlet valve of an adjacent chamber in a direction the fluid flows. - View Dependent Claims (2, 3, 4, 5)
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6. An apparatus, for use in amplifying an amount of a nucleic acid present in a sample using a polymerase chain reaction, comprising three chambers, each chamber comprising:
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an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber; and
an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber;
wherein the chambers are sequentially connected such that the outlet valve of one chamber is connected to the inlet valve of an adjacent chamber in a direction the fluid flows; and
wherein the three chambers include a first chamber maintained at a temperature for denaturing, a second chamber maintained at a temperature for annealing, and a third chamber maintained at a temperature for extension.
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7. An apparatus, for use in amplifying an amount of a nucleic acid present in a sample using a polymerase chain reaction, comprising two chambers, each chamber comprising:
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an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber; and
an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber;
wherein the outlet valve of one chamber is connected to the inlet valve of the other chamber; and
wherein one chamber is maintained at a temperature for denaturing and the other chamber is maintained at a temperature for both annealing and extension.
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8. An apparatus for circulating a carrier fluid, comprising:
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a micro-channel having two or more sections maintained at different temperatures, one section retaining a sample fluid and the remaining one or more sections retaining a magnetic fluid;
an inlet/outlet valve connected to the micro-channel; and
a magnet disposed outside the micro-channel, forming a magnetic field to effect on the magnetic fluid. - View Dependent Claims (9, 10, 11)
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12. An apparatus for use in amplifying an amount of a nucleic acid present in a sample using a polymerase chain reaction, the apparatus comprising:
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a micro-channel having three sections, one section retaining a sample fluid and the remaining sections retaining a magnetic fluid;
an inlet/outlet valve connected to the micro-channel; and
a magnet disposed outside the micro-channel, forming a magnetic field to effect on the magnetic fluid, wherein the three sections include a first section maintained at a temperature for denaturing, a second section maintained at a temperature for annealing, and a third section maintained at a temperature for extension. - View Dependent Claims (14, 15, 16, 17)
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13. An apparatus for use in amplifying an amount of a nucleic acid present in a sample using a polymerase chain reaction, the apparatus comprising:
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a micro-channel having two sections, one section retaining a sample fluid and the other section retaining a magnetic fluid;
an inlet/outlet valve connected to the micro-channel;
a magnet disposed outside the micro-channel, forming a magnetic field to effect on the magnetic fluid, wherein one section is maintained at a temperature for denaturing and the other section is maintained at a temperature for both annealing and extension.
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Specification