Reagents and methods for detecting analytes, and devices comprising reagents for detecting analytes
First Claim
Patent Images
1. A reagent for detecting an analyte, comprising:
- an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and
a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof.
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Abstract
Reagents for detecting an analyte are described. A reagent comprises (a) an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and (b) a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof. In addition, reagents having good stability to radiation sterilization are described. Electrochemical sensors and sampling devices comprising such reagents, methods of producing a sterilized device including such reagents, and methods for detecting an analyte which utilize such reagents are described as well.
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Citations
46 Claims
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1. A reagent for detecting an analyte, comprising:
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an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and
a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A reagent for detecting glucose, comprising:
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PQQ-glucose dehydrogenase in an activity of about 20 Units/μ
L;
a buffer having a concentration between about 0.1 mM and about 100 mM, and a pH between about 4.5 and about 9.5; and
a mediator having a structure wherein the mediator has a concentration in the buffer between about 0.1 mM and about 30 mM. - View Dependent Claims (15, 16, 17)
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18. A reagent for detecting an analyte, comprising:
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an enzyme selected from the group consisting of PQQ-glucose dehydrogenase, FAD-glucose oxidase, and a combination thereof;
a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and combinations thereof. - View Dependent Claims (19, 20)
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21. An electrochemical sensor comprising:
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a working electrode having a surface; and
a second electrode coupled to the working electrode;
whereinthe surface of the working electrode is coated with a solution of a reagent comprising an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof; and
a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof. - View Dependent Claims (22, 23, 24, 25)
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26. An electrochemical sensor comprising:
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a working electrode having a surface; and
a reference electrode coupled to the working electrode;
whereinthe surface of the working electrode is coated with a solution of a reagent comprising PQQ-glucose dehydrogenase in an activity of about 1 Units/μ
L to about 100 Units/μ
L;
a buffer having a concentration of about 100 mM and a pH of about 7.4; and
a mediator having a structure wherein the mediator has a concentration in the buffer of about 1 mM to about 100 mM. - View Dependent Claims (27, 28, 29)
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30. A device for measuring an analyte, comprising:
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a lancet; and
a sampling chamber connected to the lancet, comprising;
a reagent comprising;
an enzyme selected from the group consisting of PQQ-glucose dehydrogenase, FAD-glucose oxidase, and a combination thereof; and
a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof. - View Dependent Claims (31, 32, 33, 34)
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35. A method of producing a sterilized device for measuring an analyte, comprising:
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providing a device comprising;
a reagent, comprising;
an enzyme selected from the group consisting of PQQ-glucose dehydrogenase, FAD-glucose oxidase, and a combination thereof; and
a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof; and
irradiating the device with E-beam or gamma ray irradiation.
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36. A method of producing a sterilized device for measuring an analyte, comprising:
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providing a device comprising;
a lancet; and
a sampling chamber connected to the lancet, comprising;
a reagent, comprising;
an enzyme selected from the group consisting of PQQ-glucose dehydrogenase, FAD-glucose oxidase, and a combination thereof; and
a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof; and
irradiating the device with E-beam or gamma ray irradiation.
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37. A method for detecting an analyte which undergoes a chemical reaction, the method comprising:
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providing an electrode surface;
catalyzing the chemical reaction with an enzyme selected from the group consisting of a flavoprotein, a quinoprotein, and a combination thereof;
generating a redox equivalent by the chemical reaction; and
transferring the redox equivalent to the electrode surface using a mediator selected from the group consisting of a phenothiazine, a phenoxazine, and a combination thereof. - View Dependent Claims (38, 39, 40, 41, 42, 43, 44, 45, 46)
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Specification