Fluorescent quenching detection reagents and methods
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Abstract
Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
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Citations
31 Claims
- 1. An oligonucleotide probe compound having the formula:
- 2. A quencher-phosphoramidite reagent compound having the formula:
- 16. An oligonucleotide probe compound having the formula:
- 17. A quencher-phosphoramidite reagent compound having the formula:
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23. A probe compound-for a hybridization assay comprising the formula FL-ODN-(W)d-Q wherein
FL is a fluorophore with an emission wavelength in the range of about 300 to about 800 nm; -
ODN is an oligonucleotide;
W is a linker group that has from 3 to 100 atoms other than hydrogen atoms, selected from C, N, O, S, P and Si, and is cyclic, acyclic, aromatic or a combination thereof, and d is 0 or 1; and
Q is a quencher moiety comprising the structure —
Ar1—
N═
N—
Ar2 wherein Ar1 is joined to either ODN or W, and Q has a broader absorbance range than dabcyl (4-{[4-(dimethylamino)phenyl]diazenyl}benzoyl, absorbance max=453 nm), wherein Ar1 and Ar2 are each independently a substituted or unsubstituted aryl group. - View Dependent Claims (24, 26, 27, 28, 29)
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- 25. A compound of the formula
Specification