Compositions useful as ligands for the formyl peptide receptor like 1 receptor and methods of use thereof
First Claim
1. An isolated protein or polypeptide comprising a N-terminal sequence having at least 80% identity to SEQ ID NO:
- 1, excluding the sequence of SEQ ID NO;
16.
1 Assignment
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Accused Products
Abstract
The inventors have discovered that a CKβ8-1 truncation variant, CKβ8-1 (25-116), is a bifunctional ligand for two distinct GPCRs, chemokine receptor CCR1 and formyl peptide receptor like 1 (FPRL1). Hence, the inventors have discovered that, in addition to its functional activity on CCR1, CKβ8-1 (25-116) is also a functional ligand for the GPCR receptor FPRL1 that is involved in inflammatory reactions and innate immunity by recruiting monocytes and neutrophils. In addition, the inventors have discovered an alternatively spliced exon of CKβ8-1, named SHAAGtide. SHAAGtide, along with its parent chemokine CKβ8-1 (25-116), is fully functional on both monocytes and neutrophils that are known to express FPRL1.
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Citations
24 Claims
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1. An isolated protein or polypeptide comprising a N-terminal sequence having at least 80% identity to SEQ ID NO:
- 1, excluding the sequence of SEQ ID NO;
16. - View Dependent Claims (2, 3, 4, 5, 6, 19, 20, 21)
- 1, excluding the sequence of SEQ ID NO;
- 7. An isolated polypeptide at least 80% identity to SEQ ID NO:
- 11. An isolated nucleic acid comprising a nucleic acid sequence comprising at least 80% identity with SEQ ID NO:
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22. A method of identifying a FPRL1 receptor antagonist comprising:
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contacting a cell expressing a FPRL1 receptor with a protein or polypeptide comprising a N-terminal sequence having at least 80% identity to SEQ ID NO;
1;
wherein the receptor is stimulated;
contacting the receptor with a candidate antagonist compound; and
detecting to the FPRL1 receptor. - View Dependent Claims (23, 24)
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Specification