Multiaperture sample positioning and analysis system
First Claim
Patent Images
1. A method for analyzing a plurality of membranous samples, such as cells, vesicles, and cellular organelles, and fragments, derivatives, and mixtures thereof, comprising:
- selecting a measuring arrangement, comprising (i) a holder, and (ii) a plurality of measurement sites disposed in the holder, each measurement site having a carrier that separates from one another two fluid compartments, the carrier containing an aperture that connects the two compartments, wherein each carrier is adapted to seal a sample across the aperture, and wherein each compartment is in electrical contact with at least one electrode;
sealing a first sample across a first aperture in the measuring arrangement;
measuring, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of the first sample, after the first sample is sealed across the first aperture. sealing a second sample across a second aperture in the measuring arrangement; and
measuring, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of the second sample, after the second sample is sealed across the second aperture.
4 Assignments
0 Petitions
Accused Products
Abstract
Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof, for electrical and/or optical analysis, especially relating to the presence and/or activity of ion channels.
152 Citations
63 Claims
-
1. A method for analyzing a plurality of membranous samples, such as cells, vesicles, and cellular organelles, and fragments, derivatives, and mixtures thereof, comprising:
-
selecting a measuring arrangement, comprising (i) a holder, and (ii) a plurality of measurement sites disposed in the holder, each measurement site having a carrier that separates from one another two fluid compartments, the carrier containing an aperture that connects the two compartments, wherein each carrier is adapted to seal a sample across the aperture, and wherein each compartment is in electrical contact with at least one electrode;
sealing a first sample across a first aperture in the measuring arrangement;
measuring, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of the first sample, after the first sample is sealed across the first aperture. sealing a second sample across a second aperture in the measuring arrangement; and
measuring, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of the second sample, after the second sample is sealed across the second aperture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
-
-
30. A method for analyzing membranous samples, such as cells, vesicles, and cellular organelles, and fragments, derivatives, and mixtures thereof, comprising:
-
selecting a measuring arrangement, comprising (i) a carrier having a plurality of apertures, (ii) at least two fluid compartments, separated by the carrier, each fluid compartment connected to at least one of the other fluid compartments via at least one of the apertures, and (iii) at least two electrodes, each electrode being in electrical contact with at least one of the fluid compartments;
sealing a first sample across a first aperture selected from the plurality of apertures, and sealing a second sample across a second aperture selected from the plurality of apertures; and
measuring, distinguishably, at least one of an electrical potential and a current across at least a portion of each of the first and second samples, following the step of sealing the samples across the apertures. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57)
-
-
58. A method for analyzing membranous samples, such as cells, vesicles, and cellular organelles, and fragments, derivatives, and mixtures thereof, comprising:
-
selecting a holder having a plurality of measurement sites, each measurement site having a carrier containing an aperture, wherein fluid is disposed on opposite sides of the aperture;
sealing a first sample across an aperture at a first of the plurality of measurement sites, and a second sample across an aperture at a second of the plurality of measurement sites; and
measuring, distinguishably, at least one of an electrical potential difference and a current across at least a portion of each off the first and second samples, following the step of sealing the samples across the apertures. - View Dependent Claims (59, 60, 61, 62)
-
-
63. A method for analyzing membranous samples, such as cells, vesicles, and cellular organelles, and fragments, derivatives, and mixtures thereof, comprising:
-
selecting a measuring arrangement, comprising (i) a carrier having a plurality of apertures, the carrier being adapted so that at least one of the samples can be sealed across at least one of the apertures, (ii) at least one sample fluid compartment and at least one reference fluid compartment, in contact via the apertures, and (iii) at least one sample electrode and at least one reference electrode, each sample electrode in contact with at least one of the sample fluid compartments, and each reference electrode in contact with at least one of the reference fluid compartments, wherein the at least one sample electrode and the at least one reference electrode are adapted to apply and/or measure an electrical potential across the apertures;
sealing a first sample across a first aperture in the measuring arrangement, and sealing a second sample across a second aperture in the measuring arrangement; and
measuring, distinguishably, using at least two of the electrodes, at least one of an electrical potential and a current across at least a portion of each of the first and second samples, following the step of sealing the samples across the apertures.
-
Specification