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System and method for detecting and classifying biological particles

  • US 20030098422A1
  • Filed: 11/07/2002
  • Published: 05/29/2003
  • Est. Priority Date: 11/07/2001
  • Status: Active Grant
First Claim
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1. A system for detecting and classifying a biological particle, comprising:

  • a laser to illuminate the biological particle;

    a first detector to detect light scattered due to illumination of the biological particle by the laser;

    a second detector to detect light emitted if the biological particle auto-fluoresces due to illumination of the biological particle by the laser;

    a processor to determine a first peak or integrated pulse amount of light detected by the first detector, a second peak or integrated pulse amount of light detected by the second detector, and to calculate a ratio of the first and second peak or integrated pulse and to compare to a library comprising a plurality of ratios of scattering peaks to auto-fluorescence peaks; and

    wherein the processor compares the calculated ratio with the plurality of ratios stored in the library to classify the biological particle.

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