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Nucleic acid amplification

  • US 20030099937A1
  • Filed: 08/15/2002
  • Published: 05/29/2003
  • Est. Priority Date: 08/15/2001
  • Status: Abandoned Application
First Claim
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1. A method of producing RNA replicates, the method comprising:

  • providing a first solid support having attached oligonucleotides that comprise a promoter sequence and a target binding sequence;

    annealing a sample that comprises RNAs to the solid support;

    extending the attached oligonucleotides using an RNA-directed DNA polymerase to construct DNA replicates of the RNAs;

    synthesizing DNA strands complementary to the DNA replicates, thereby producing a double-stranded template that includes the promoter sequence;

    joining an adaptor that comprises a tag sequence to the double-stranded template, and transcribing the double-stranded template using an RNA polymerase that recognizes the promoter region to produce first-stranded RNA replicates.

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