Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
First Claim
Patent Images
1. A photocleavable marker-polynucleotide conjugate having the formula:
1 Assignment
0 Petitions
Accused Products
Abstract
Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.
52 Citations
35 Claims
- 1. A photocleavable marker-polynucleotide conjugate having the formula:
- 13. A photocleavable marker-polynucleotide conjugate having the formula:
-
25. A method of preparing a marker-photocleavable linker-nucleotide conjugate comprising:
-
a) providing i) a photocleavable linker comprising a protective group, ii) a nucleotide, and iii) an activated marker molecule;
b) operably linking said photocleavable linker to said nucleotide to produce a photocleavable linker-nucleotide conjugate;
c) removing said protective group from said photocleavable linker-nucleotide conjugate under conditions such that an activated photocleavable linker-nucleotide conjugate is created, wherein said activated photocleavable linker-nucleotide conjugate has an exposed reactive site on the linker portion of the conjugate; and
d) contacting said activated marker molecule with said activated photocleavable linker-nucleotide conjugate under conditions such that a marker-photocleavable linker-nucleotide conjugate is produced. - View Dependent Claims (26, 27, 28)
-
-
29. A method of preparing photocleavable marker-polynucleotide conjugates comprising:
-
a) providing i) an unmodified polynucleic acid, ii) a photocleavable marker nucleotide, and iii) a nucleic acid-modifying enzyme;
b) contacting said polynucleic acid with said photocleavable marker nucleotide and said modifying enzyme under conditions such that said photocleavable marker nucleotide is incorporated into said polynucleic acid to produce a labeled polynucleic acid. - View Dependent Claims (30, 31, 32, 33, 34, 35)
-
Specification