Biosensor and method for its preparation
First Claim
1. A biosensor which is provided with a development layer for developing an inspection target solution, includes a region of an immobilized reagent which is immobilized in a part of the development layer and a region of a marked reagent which is held in a part of the development layer in a marked dry state to be eluted by development of the inspection target solution, and measures a bonding amount of the marked reagent in the immobilized reagent region, thereby qualitatively or quantitatively measuring a measurement component in the inspection target solution, wherein side faces of the development layer which are parallel to the direction of the inspection target solution permeating are partially or entirely melted and cured to be sealed.
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Accused Products
Abstract
A biosensor according to the present invention includes a reagent immobilization part (5) where an antibody for a measurement target in an inspection target solution is immobilized, and a marked reagent holding part (4) where an antibody which is marked at a part of a development layer in a dry state to be eluted by development of the inspection target solution is held, at parts of the development layer for developing the inspection target solution, and measures a bonding amount of the marked reagent bonded in the reagent immobilization part (5), thereby qualitatively and quantitatively measuring a measurement component in the inspection target solution. In such bionsensor, side faces of the development layer which are parallel to the direction of the inspection target solution permeating are partially or entirely melted and cured to be sealed.
According to the so-constituted biosensor, the permeation of the inspection target solution in the development layer is arranged, and a low-cost and high-accuracy biosensor and a manufacturing method thereof can be realized by a simplified biosensor manufacturing method.
48 Citations
21 Claims
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1. A biosensor which is provided with a development layer for developing an inspection target solution, includes a region of an immobilized reagent which is immobilized in a part of the development layer and a region of a marked reagent which is held in a part of the development layer in a marked dry state to be eluted by development of the inspection target solution, and measures a bonding amount of the marked reagent in the immobilized reagent region, thereby qualitatively or quantitatively measuring a measurement component in the inspection target solution, wherein
side faces of the development layer which are parallel to the direction of the inspection target solution permeating are partially or entirely melted and cured to be sealed.
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2. A biosensor which is provided with a development layer for developing an inspection target solution, includes a region of an immobilized reagent which is immobilized in a part of the development layer that includes its side faces parallel to the direction of the inspection target solution permeating and a region of a marked reagent which is held in a part of the development layer that includes its side faces parallel to the direction of the inspection target solution permeating, in a marked dry state to be eluted by development of the inspection target solution, and measures a bonding amount of the marked reagent in the immobilized reagent region, thereby qualitatively or quantitatively measuring a measurement component in the inspection target solution, wherein
a reagent component on the side faces of the development layer parallel to the inspection target solution permeating, in the marked reagent region and the immobilized reagent region is denatured to be deactivated.
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12. A method for manufacturing a biosensor which is provided with a development layer for developing an inspection target solution, includes a region of an immobilized reagent which is immobilized in a part of the development layer and a region of a marked reagent which is held in a part of the development layer in a marked dry state to be eluted by development of the inspection target solution, and measures a bonding amount of the marked reagent in the immobilized reagent region, thereby qualitatively or quantitatively measuring a measurement component in the inspection target solution, including
a melting process for melting and curing side faces of the development layer which are parallel to the direction of the inspection target solution permeating to seal the same partially or entirely.
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13. The biosensor manufacturing as defined in 12, wherein
the melting process is implemented by irradiating a laser to the side faces of the development layer partially or entirely.
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14. A method for manufacturing a biosensor which is provided with a development layer for developing an inspection target solution, includes a region of an immobilized reagent which is immobilized in a part of the development layer and a region of a marked reagent which is held in a part of the development layer in a marked dry state to be eluted by development of the inspection target solution, and measures a bonding amount of the marked reagent in the immobilized reagent region, thereby qualitatively or quantitatively measuring a measurement component in the inspection target solution, including
a cutting and melting process for cutting the sheet-shaped development layer in parallel to the direction of the inspection target solution permeating, and simultaneously melting and curing cut surfaces of the development layer to seal.
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16. A method for manufacturing a biosensor which is provided with a development layer for developing an inspection target solution, includes a region of an immobilized reagent which is immobilized in a part of the development layer that includes its side faces parallel to the direction of the inspection target solution permeating and a region of a marked reagent which is held in a part of the development layer that includes its side faces parallel to the direction of the inspection target solution permeating, in a marked dry state to be eluted by development of the inspection target solution, and measures a bonding amount of the marked reagent in the immobilized reagent region, thereby qualitatively or quantitatively measuring a measurement component in the inspection target solution, including
a denaturing and deactivating process for denaturing and deactivating a reagent component on the side faces parallel to the inspection target solution permeating, in the marked reagent region and the immobilized reagent region.
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18. A method for manufacturing a biosensor which is provided with a development layer for developing an inspection target solution, includes a region of an immobilized reagent which is immobilized in a part of the development layer that includes its side faces parallel to the direction of the inspection target solution permeating and a region of a marked reagent which is held in a part of the development layer that includes its side faces parallel to the inspection target solution permeating, in a marked dry state to be eluted by development of the inspection target solution, and measures a bonding amount of the marked reagent in the immobilized reagent region, thereby qualitatively or quantitatively measuring a measurement component in the inspection target solution, including
a melting and deactivating process for melting and curing side faces of the development layer which are parallel to the direction of the inspection target solution permeating to seal the same partially or entirely, as well as denaturing and deactivating a reagent component on the side faces parallel to the inspection target solution permeating, in the marked reagent region and the immobilized reagent region.
- 20. A method for manufacturing a biosensor which is provided with a development layer for developing an inspection target solution, includes a region of an immobilized reagent which is immobilized in a part of the development layer that includes its side faces parallel to the direction of the inspection target solution permeating and a region of a marked reagent which is held in a part of the development layer that includes its side faces parallel to the inspection target solution permeating, in a marked dry state to be eluted by development of the inspection target solution, and measures a bonding amount of the marked reagent in the immobilized reagent region, thereby qualitatively or quantitatively measuring a measurement component in the inspection target solution, including a cutting, melting and deactivating process for cutting the sheet-shaped development layer in parallel to the direction of the inspection target solution permeating, simultaneously melting and curing cut surfaces of the development layer to seal, and denaturing and deactivating a reagent component on the side faces in the marked reagent region and the immobilized reagent region.
Specification