Membrane transportable fluorescent substrates
First Claim
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1. A fluorescent substrate delivery system comprising a complex of an enzyme active fluorescent substrate and a carrier molecule.
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Abstract
Intracellular enzyme-activated fluorescent substrates that can be transported into a cell are provided. The membrane transportable fluorescent substrates are complexes (e.g., ionic complexes) formed between an enzyme activated fluorescent substrate and a carrier molecule. The fluorescent substrates can be used in an intracellular assay of enzyme activity and/or expression.
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Citations
42 Claims
- 1. A fluorescent substrate delivery system comprising a complex of an enzyme active fluorescent substrate and a carrier molecule.
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12. A method for detecting the presence and/or measuring the activity of an enzyme in a cell comprising:
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contacting a sample comprising the cell with a composition comprising a complex of a carrier molecule and an enzyme active fluorescent substrate comprising a group cleavable by the enzyme; and
detecting fluorescence from the sample;
wherein the presence of fluorescence indicates the presence of the enzyme in the cell and wherein the intensity of fluorescence indicates the activity or expression of the enzyme in the cell. - View Dependent Claims (13, 14, 15, 16)
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- 17. A fluorescent substrate comprising at least one ionic group available for complexation through ionic charge interactions with oppositely charged ionic groups on a carrier molecule.
Specification