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Real-time gene quantification with internal standards

  • US 20030104438A1
  • Filed: 08/29/2002
  • Published: 06/05/2003
  • Est. Priority Date: 08/31/2001
  • Status: Active Grant
First Claim
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1. A method of determining mass fractions of first and second target nucleic acids present in a test sample, said method comprising the steps of (a) contacting the target nucleic acids with a fluorescent nucleic acid indicator, the indicator being configured to provide a signal related to the quantity of indicator hybridized to the target nucleic acid, the indicator further configured to discriminate the target nucleic acids based on melting temperature, (b) illuminating the test sample, (c) monitoring fluorescent change to generate a melting curve, and (d) using a thermodynamically based signal processing algorithm to determine the mass fraction of the target nucleic acids.

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