Biosensors for single cell and multi cell analysis
First Claim
1. A structure comprising:
- a substrate having a microfluidic channel;
a substrate membrane disposed over the substrate and in fluidic connection with the microfluidic channel, and further having at least one opening operable to allow passage of molecules having a set of predetermined characteristics;
first and second electrodes disposed on both sides of the substrate membrane operable to detect molecular transport across the substrate membrane and a biological substance deposited thereon.
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Abstract
The present invention relates to a structure comprising a biological membrane and substrate with fluidic network, an array of membranes and an array of fluidic networks in substrate, a high throughput screen, methods for production of the membrane, substrate structure, and a method for interconnected array of substrate structures and a method for attaching membranes to structure, a method to electrically record events from the membranes and a method to screen large compound library using the array. More particularly, it relates to biological cells and artificial cell membranes adhered to the substrate with a high electrical resistivity seal, a method to manufacture array configuration of such substrates, and a method to screen compounds using the membrane receptors such as ion-channels, ion pumps, & receptors.
159 Citations
51 Claims
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1. A structure comprising:
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a substrate having a microfluidic channel;
a substrate membrane disposed over the substrate and in fluidic connection with the microfluidic channel, and further having at least one opening operable to allow passage of molecules having a set of predetermined characteristics;
first and second electrodes disposed on both sides of the substrate membrane operable to detect molecular transport across the substrate membrane and a biological substance deposited thereon. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 51)
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23. A method, comprising:
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dispensing biological substance on a substrate membrane having predefined porosity, the substrate membrane being disposed over a substrate having a microfluidic channel in fluidic communication with the substrate membrane;
applying an electrical potential across the substrate membrane; and
detecting and measuring a charged species flux across the substrate membrane. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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45. A method of forming, comprising:
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chemically depositing a thin film of thermal oxide and nitride on both sides with a first side of a substrate having an electronic circuit;
patterning the thin film on the second side of the substrate and using this patterned thin film as a mask;
creating an opening on the second side of the substrate to form a suspended thin film membrane on the first side allowing transmission of light there through;
forming an electrode pattern on the first side, the electrode pattern being coupled to the electronic circuit.
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46. The method as claimed in 45, forming prior to forming an electrode pattern further comprising;
forming a second substrate on the patterned second side of the first substrate, thereby creating a microfluidic channel in fluid communication with the suspended membrane. - View Dependent Claims (49, 50)
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47. The method as claimed in 45:
forming a second substrate on the patterned second side of the first substrate, thereby creating a microfluidic channel in fluid communication with the suspended membrane.
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48. The method as claimed in 45:
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forming an electrode pattern on the second side;
forming a second substrate on the patterned second side of the first substrate, thereby creating a microfluidic channel in fluid communication with the suspended membrane.
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Specification