Method for analysing a diffusing sample by time resolution measurement

• US 20030107742A1
• Filed: 10/31/2002
• Published: 06/12/2003
• Est. Priority Date: 05/18/2000
• Status: Active Grant

• Current Assignee: Université Paris 13
• Original Assignee: Université Paris 13
• Inventors: Tualle, Jean-Michel
• Granted Patent: US 6,903,825 B2
• Time in Patent Office: Days
• US Class Current: 356/450
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B

Abstract

The invention concerns a method for analysing a diffusing sample comprising steps which consist in: a) illuminating the sample with a temporally coherent and wavelength-modulated incident light beam; b) producing a succession of interference signals with the diffuse light; c) combining each interference signal with a reference signal (Ref(t,τ₀)) generating a time port; d) extracting the continuous component of each signal characteristic of the predetermined delay (τ₀)) derived form c); applying a non-linear function on each of the continuous components of the signals characterising the predetermined delay (τ₀); and f) averaging each of the images, with said non-linear function, of the continuous components of the signals characterising the predetermined delay (τ₀). The invention is useful for observing organs of the human body.

Claims

1. A method of analyzing a scattering sample by time resolved measurement of the light scattered within this sample, characterized in that it comprises the steps of:

a) illuminating the sample with a temporally coherent and wavelength-modulated incident light beam;

b) producing a succession of interference signals, recorded over a time interval, by superposition of the scattered light obtained at the output of the sample and of light taken from the incident beam illuminating the sample;

c) combining each interference signal with a reference signal (Ref(t,τ

₀)) generating a time gate centered on a predetermined delay (τ

₀) in order to produce a signal characteristic of the predetermined delay (τ

₀);

d) extracting the d.c. component of each signal characteristic of the predetermined delay (τ

₀);

e) applying a nonlinear function to each of the d.c. components of the signals characteristic of the predetermined delay (τ

₀); and

f) carrying out a linear combination of each of the nonlinear function images of the d.c. components of the signals characteristic of the predetermined delay (τ

₀).

2. The method as claimed in claim 1, characterized in that said nonlinear function is a function chosen from the group consisting of square functions and the absolute value function.

3. The method as claimed in claim 1, characterized in that said nonlinear function is a function of at least two variables taken from the d.c. components of the signals characteristic of the predetermined delay (τ

₀) obtained from the succession of interference signals recorded over the time interval.

4. The method as claimed in any one of the preceding claims, characterized in that, for the combination of each interference signal with said reference signal (Ref(t,τ

₀)), the incident beam is additionally amplitude modulated by means of an amplitude modulation introducing the predetermined delay (τ

₀).

5. The method as claimed in claim 4, characterized in that, for an amplitude modulation equal to the sum of said reference signal (Ref(t,τ

₀)) and of a reference signal with zero delay (Ref(t,0), said linear combination comprises a calculation subtracting from each of the nonlinear function images of d.c. components of the signals characteristic of a zero delay (τ

₀=0).

6. The method as claimed in any one of the preceding claims, characterized in that said linear combination comprises calculating the mean of each of the nonlinear function images of the d.c. components of the signals characteristic of the predetermined delay (τ

₀).

7. The method as claimed in any one of the preceding claims, characterized in that, when producing the succession of interference signals, a relative displacement is imposed between the incident beam and the scattering sample, the frequency of the imposed displacement being less than the frequency of the wavelength modulation of the light of the incident beam.

8. The method as claimed in any one of the preceding claims, characterized in that said reference signal (Ref(t,τ

₀)) comprises a sinusoidal function of the product of the predetermined delay (τ

₀) on which the time gate is centered and of the wavelength modulation function of the incident beam.

9. An apparatus for analyzing a scattering sample by time resolved measurement of the light scattered within this sample, characterized in that it comprises:

a) means (16, 18;

100, 104, 102, 106) for illuminating the sample with a temporally coherent and wavelength-modulated incident light beam;

b) an interferometer (11;

109) for producing a succession of interference signals, recorded over a time interval, by superposition of the scattered light obtained at the output of the sample and of light taken from the incident beam illuminating the sample;

c) means (52, 54) for combining each interference signal with a reference signal (Ref(t,τ

₀)) generating a time gate centered on a predetermined delay (τ

₀) in order to produce a signal characteristic of the predetermined delay (τ

₀);

d) means (56) for extracting the d.c. component of each signal characteristic of the predetermined delay (τ

₀) ;

e) means (58) for applying a nonlinear function to each of the d.c. components of the signals characteristic of the predetermined delay (τ

₀); and

f) means (60) for carrying out a linear combination of each of the nonlinear function images of the d.c. components of the signals characteristic of the predetermined delay (τ

₀).

10. Equipment for measuring the content of a particular component in part of a living organism, comprising an apparatus for analyzing a scattering sample according to claim 9, and means (132) for calculating said content of a particular component as a function of the result of the analysis supplied by said apparatus.

11. The equipment as claimed in claim 10, characterized in that said analysis apparatus is suitable for producing interference signals from the light backscattered by an organ of a human being or of an animal, and in that said calculating means are suitable for calculating the oxygenation level of this organ.

12. Imaging equipment comprising:

an analysis apparatus according to claim 9, apparatus which is suitable for producing and processing several successions of interference signals obtained from the light scattered in several adjacent regions of the sample and thus to supply an analysis result for each of the adjacent regions of the sample; and

means (156) for producing an image of the sample from the analysis result supplied for each of the adjacent regions of the sample.

13. The equipment as claimed in claim 12, characterized in that said analysis apparatus is suitable for producing interference signals from the scattered light transmitted through an organ of a human being or of an animal.