×

Nucleic acid sequencing using microsphere arrays

  • US 20030108867A1
  • Filed: 02/25/2000
  • Published: 06/12/2003
  • Est. Priority Date: 04/20/1999
  • Status: Abandoned Application
First Claim
Patent Images

1. A method of sequencing a plurality of target nucleic acids each comprising a first domain and a adjacent second domain, said second domain comprising a plurality of target positions, said method comprising:

  • a) providing a plurality of hybridization complexes each comprising a target sequence and a sequencing primer that hybridizes to the first domain of said target sequence, said hybridization complexes attached to a surface of a substrate;

    b) extending each of said primers by the addition of a first nucleotide to the first detection position using a first enzyme to form an extended primer; and

    c) detecting the release of pyrophosphate (PPi) to determine the type of said first nucleotide added onto said primers.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×