Melanocortin-5 receptor sequences and uses thereof
First Claim
Patent Images
1. An isolated nucleic acid encoding a functional MC5R, or the complement thereof, said nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence which hybridizes under conditions of moderate stringency to the coding region of SEQ ID NO;
1;
(b) a nucleotide sequence which hybridizes under conditions of moderate stringency to a polynucleotide which is complementary to the coding region of SEQ ID NO;
1;
(c) a nucleotide sequence which hybridizes under conditions of moderate stringency to the coding region of the canine MC5R as deposited with the ATCC and having ATCC Accession No. PTA-3455; and
(d) a nucleotide sequence which hybridizes under conditions of moderate stringency to a polynucleotide which is complementary to the coding region of the canine MC5R as deposited with the ATCC and having ATCC Accession No. PTA-3455, with the proviso that said functional MC5R is not human, bovine, chimpanzee, murine, rat, chicken or swine.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to nucleic acids encoding functional canine melanocortin 5 receptors and their polypeptide products. The present invention further relates to screening assays to identify compounds that modulate the activity or expression of the melanocortin 5 receptor of the invention. The present invention also relates to methods and therapeutic compositions for the treatment of inappropriate food intake and skin disorders comprising administering to animals compounds that modulate the activity or expression of melanocortin 5 receptors.
29 Citations
33 Claims
-
1. An isolated nucleic acid encoding a functional MC5R, or the complement thereof, said nucleic acid comprising a nucleotide sequence selected from the group consisting of:
-
(a) a nucleotide sequence which hybridizes under conditions of moderate stringency to the coding region of SEQ ID NO;
1;
(b) a nucleotide sequence which hybridizes under conditions of moderate stringency to a polynucleotide which is complementary to the coding region of SEQ ID NO;
1;
(c) a nucleotide sequence which hybridizes under conditions of moderate stringency to the coding region of the canine MC5R as deposited with the ATCC and having ATCC Accession No. PTA-3455; and
(d) a nucleotide sequence which hybridizes under conditions of moderate stringency to a polynucleotide which is complementary to the coding region of the canine MC5R as deposited with the ATCC and having ATCC Accession No. PTA-3455, with the proviso that said functional MC5R is not human, bovine, chimpanzee, murine, rat, chicken or swine. - View Dependent Claims (9, 10, 11, 12, 18, 19, 20, 21, 22, 23, 24, 25, 33)
-
-
2. An isolated nucleic acid encoding a functional MC5R, or the complement thereof, said nucleic acid comprising a nucleotide sequence selected from the group consisting of:
-
(a) a nucleotide sequence which hybridizes under conditions of high stringency to the coding region of SEQ ID NO;
1;
(b) a nucleotide sequence which hybridizes under conditions of high stringency to a polynucleotide which is complementary to the coding region of SEQ ID NO;
1;
(c) a nucleotide sequence which hybridizes under conditions of high stringency to the coding region of the canine MC5R as deposited with the ATCC and having ATCC Accession No. PTA-3455; and
(d) a nucleotide sequence which hybridizes under conditions of high stringency to a polynucleotide which is complementary to the coding region of the canine MC5R as deposited with the ATCC and having ATCC Accession No. PTA-3455.
-
-
3. An isolated nucleic acid comprising a nucleotide sequence that:
-
(a) encodes a polypeptide according to SEQ ID NO;
2;
or(b) encodes a polypeptide encoded by the canine MC5R clone as deposited with the ATCC and having ATCC Accession No. PTA-3455. - View Dependent Claims (4)
-
-
5. An isolated nucleic acid comprising a nucleotide sequence having more than about 87% identity to SEQ ID NO:
- 3 or to the canine MC5R clone as deposited with the ATCC and having ATCC Accession No. PTA-3455.
-
6. An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide having more than about 87% identity to SEQ ID NO:
- 2 or to the polypeptide encoded by the canine MC5R clone as deposited with the ATCC and having ATCC Accession No. PTA-3455.
-
7. An isolated nucleic acid comprising a nucleotide sequence encoding an extracellular domain of a canine MC5R corresponding to amino acids 1-37, 90-119, 181-185, or 265-272 of SEQ ID NO:
- 2 or of the polypeptide encoded by the canine MC5R clone as deposited with the ATCC and having ATCC Accession No. PTA-3455.
-
8. An isolated nucleic acid comprising a nucleotide sequence encoding a cytoplasmic domain of a canine MC5R corresponding to amino acids 63-70, 139-160, 213-217, or 297-325 of SEQ ID NO:
- 2 or of the polypeptide encoded by the canine MC54R clone as deposited with the ATCC and having ATCC Accession No. PTA-3455.
-
13. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
-
(a) an amino acid sequence according to SEQ ID NO;
2;
(b) an amino acid sequence encoded by the canine MC5R clone as deposited with the ATCC and having the ATCC Accession No. PTA-3455;
(c) an extracellular domain of a canine MC5R corresponding to amino acids 1-37, 90-119, 181-185, or 265-272 of SEQ ID NO;
2 or of the polypeptide encoded by the canine MC5R clone as deposited with the ATCC and having ATCC Accession No. PTA-3455; and
(d) a cytoplasmic domain of a canine MC5R corresponding to amino acids 63-70, 139-160, 213-237, or 297-325 of SEQ ID NO;
2 or of the polypeptide encoded by the canine MC5R clone as deposited with the ATCC and having ATCC Accession No. PTA-3455.
-
-
14. An antibody that immuno-specifically binds a polypeptide having the amino acid sequence of SEQ ID NO:
- 2.
-
15. A method for producing a recombinant canine MC5R polypeptide, comprising:
-
(a) culturing a host cell transformed with an expression vector which expresses the recombinant canine MC5R polypeptide; and
(b) recovering the recombinant canine MC5R polypeptide from the cell culture.
-
-
16. A method for isolating a recombinant canine MC5R polypeptide, comprising:
-
(a) culturing a host cell transformed with an expression vector which expresses the recombinant canine MC5R polypeptide in the host cell membranes; and
(b) isolating membranes comprising canine MC5R polypeptide from the host cell.
-
-
17. A composition comprising a substantially pure polypeptide having the amino acid sequence of SEQ ID NO:
- 2 and a carrier.
- 26. A method for modulating the appetite and/or metabolic rate of an animal in need thereof comprising administering to the animal an effective amount of an MC5R ligand.
- 30. A method of treating skin disorders in an animal comprising administering to an animal in need thereof an effective amount of an MC5R ligand.
Specification