Biosensor and method for manufacturing the same

• US 20030111344A1
• Filed: 11/14/2002
• Published: 06/19/2003
• Est. Priority Date: 05/16/2000
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Yamaoka, Hideaki
• Granted Patent: US 7,063,775 B2
• Time in Patent Office: Days
• US Class Current: 204/403.06
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: G01N 2333/904 acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

A biosensor (1) includes a capillary (3) having a channel (30) with an inlet port (30a) for taking in a sample liquid, a membrane (8) for promoting the movement of the sample liquid through the channel (30), and a reaction part (7) containing a reagent that reacts with a component to be tested in the sample liquid whose movement has been promoted by the membrane (8).

Claims

1. A biosensor, comprising:

a capillary having a channel with an inlet port for taking in a sample liquid;

a membrane for promoting movement of said sample liquid through said channel; and

a reaction part containing a reagent that reacts with a component to be tested in said sample liquid whose movement has been promoted by said membrane.

2. The biosensor according to claim 1, wherein said membrane is provided in the vicinity of said inlet port.

3. The biosensor according to claim 1, wherein said membrane has a plurality of pores each of which is no less than 0.25 μ

m but no more than 45 μ

m in diameter.

4. The biosensor according to claim 1, wherein said membrane is laminated on said reaction part.

5. The biosensor according to claim 4, wherein said reaction part and said membrane are formed to extend to said inlet port.

6. The biosensor according to claim 4, wherein said membrane is an asymmetric membrane or composite membrane comprising, thicknesswise, a first layer having smaller pores, and a second layer having larger pores, and wherein said first layer is held in contact with said reaction part.

7. The biosensor according to claim 6, wherein the pores of said first layer have a pore diameter of no less than 0.25 μ

m but no more than 0.45 μ

m, and the pores of said second layer have a pore diameter of no less than 25 μ

m but no more than 45 μ

m.

8. The biosensor according to claim 1, wherein said capillary has an opening serving as an air escape hole.

9. The biosensor according to claim 1, wherein said capillary has a transparent part or semitransparent part for internally observing said capillary.

10. The biosensor according to claim 9, wherein said membrane is white.

11. The biosensor according to claim 1, further comprising an elongate working electrode and an elongate counter electrode provided on an insulating base, wherein said capillary is formed to intersect said working electrode and said counter electrode on said insulating base.

12. The biosensor according to claim 11, wherein said counter electrode is generally parallel to said working electrode.

13. The biosensor according to claim 11, wherein said capillary comprises a pair of upright walls on said base, and a cover bridging the pair of walls.

14. A method for manufacturing a biosensor which comprises a capillary having a channel with an inlet port for taking in a sample liquid, a membrane for promoting movement of said sample liquid through said channel, and a reaction part containing a reagent that reacts with a component to be tested in said sample liquid whose movement has been promoted by said membrane;

the method comprising the steps of;

forming a working electrode and a counter electrode in an elongate configuration on an insulating base;

forming an elongate reaction part intersecting said working electrode and said counter electrode;

laminating an elongate membrane on said reaction part;

providing a pair of walls along longitudinal edges on both sides of said reaction part and said membrane; and

forming a capillary by forming a cover bridging said pair of walls.

15. The biosensor manufacturing method according to claim 14, wherein said pair of walls is formed by applying a hot melt adhesive.

16. The biosensor manufacturing method according to claim 14, wherein said pair of walls is formed by sticking double-sided tape onto said insulating base.