Methods and devices for the integrated discovery of cell culture environments
First Claim
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1. A cell culture device for assessing the effects of three-dimensional (3D) scaffolds on a desired cellular activity, comprising a culture vessel or support surface in contact with a layer of a cell adhesion resistant (CAR) material that forms a CAR surface, and a plurality of 3D scaffolds in contact with said CAR surface.
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Abstract
The present invention is directed to methods and devices which can be used to test bioactive agents alone or in conjunction with 3D scaffolds for their effect on cell growth, differentiation, and other cellular functions.
68 Citations
43 Claims
- 1. A cell culture device for assessing the effects of three-dimensional (3D) scaffolds on a desired cellular activity, comprising a culture vessel or support surface in contact with a layer of a cell adhesion resistant (CAR) material that forms a CAR surface, and a plurality of 3D scaffolds in contact with said CAR surface.
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22. A method for designing a culture system that permits or promotes a predetermined amount or level of a cellular activity, and which system optionally enables assessment of said cellular activity, said method comprising;
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(a) testing cells for said cellular activity after culture a culture vessel or on a culture surface in the presence of one or more first level combinations of (i) a candidate culture medium component; and
(ii) a candidate bioactive surface comprising one or more bioactive agents presented to cells in or on one or more 3D scaffolds;
which culture vessel surface and/or scaffold is in contact with a CAR material, thereby identifying an optimal first level combination of said medium components and said bioactive agents;
(b) selecting said optimal first level combination identified in step (a); and
(c) incorporating said selected optimal first level combination of medium components and bioactive agents into a culture system comprising a culture vessel or surface and one or more 3D scaffolds have therein or thereon said selected optimal first level combination of medium components and bioactive surface, thereby designing said culture system. - View Dependent Claims (23, 24, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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25. A method for designing a culture system that permits or promotes a predetermined amount or level of a cellular activity, and which system optionally enables assessment of said cellular activity, said method comprising;
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(a) testing cells for said activity after culture in a culture vessel or a culture surface, comprising a plurality of 3D scaffolds, in the presence of one or more combinations of (i) one or more a bioactive agents bound to one or more CAR materials on said scaffolds, and (ii) one or more culture medium components, thereby identifying an optimal combination of CAR material, bioactive agent and medium component;
(b) selecting said optimal combination identified in step (a), (c) incorporating said selected optimal combination of CAR material, medium component and bioactive agent into a culture system comprising (i) a culture vessel or surface comprising the CAR material of said combination, (ii) in contact with said culture vessel or surface, one or more 3D scaffolds having attached thereto the CAR material of said optimal combination thereby designing said culture system. - View Dependent Claims (26, 27, 28, 29)
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43. A method for producing a device for assessing the effects, on a selected cellular activity, of culturing cells in a culture system that comprises a 3D scaffold, said method comprising:
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(a) screening bioactive agents bound to a first CAR layer on a first support surface for their effect on said cellular activity by culturing cells in the presence of said agents, and selecting one or more of said agents that have a desired effect on said cellular activity;
(b) depositing said one or more bioactive agents selected in (a onto a 3D scaffold to generate bioactive surfaces on said scaffold; and
(c) depositing a plurality of said scaffolds having said bioactive surfaces onto a second CAR support surface, wherein said first and said second support surfaces comprise either the same or different CAR material.
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Specification