Antibody composition-producing cell
First Claim
1. A Chinese hamster ovary tissue-derived CHO cell into which a gene encoding an antibody molecule is introduced, which produces an antibody composition comprising an antibody molecule having complex N-glycoside-linked sugar chains bound to the Fc region, wherein among the total complex N-glycoside-linked sugar chains bound to the Fc region in the composition, the ratio of a sugar chain in which fucose is not bound to N-acetylglucosamine in the reducing end in the sugar chain is 20% or more.
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Abstract
The present invention relates to a cell for the production of an antibody molecule such as an antibody useful for various diseases having high antibody-dependent cell-mediated cytotoxic activity, a fragment of the antibody and a fusion protein having the Fc region of the antibody or the like, a method for producing an antibody composition using the cell, the antibody composition and use thereof.
364 Citations
61 Claims
- 1. A Chinese hamster ovary tissue-derived CHO cell into which a gene encoding an antibody molecule is introduced, which produces an antibody composition comprising an antibody molecule having complex N-glycoside-linked sugar chains bound to the Fc region, wherein among the total complex N-glycoside-linked sugar chains bound to the Fc region in the composition, the ratio of a sugar chain in which fucose is not bound to N-acetylglucosamine in the reducing end in the sugar chain is 20% or more.
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22. An antibody composition which comprises an antibody molecule having complex N-glycoside-linked sugar chains bound to the Fc region which is produced by a CHO cell, wherein among the total complex N-glycoside-linked sugar chains bound to the Fc region in the composition, the ratio of a sugar chain in which fucose is not bound to N-acetylglucosamine in the reducing end in the sugar chain is 20% or more.
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23. A cell in which the activity of an enzyme relating to the synthesis of an intracellular sugar nucleotide, GDP-fucose and/or the activity of an enzyme relating to the modification of a sugar chain wherein 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α
- -bond in the complex N-glycoside-linked sugar chain is decreased or deleted by a genetic engineering technique.
- View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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42. A transgenic non-human animal or plant or the progenies thereof, comprising a genome which is modified such that the activity of an enzyme relating to the synthesis of an intracellular sugar nucleotide, GDP-fucose and/or the activity of an enzyme relating to the modification of a sugar chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α
- -bond in the N-glycoside-linked sugar chain is decreased.
- View Dependent Claims (43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54)
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57. A protein selected from the group consisting of the following (a), (b), (c), (d), (e), (f), (g), (h), (i) and (j):
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(a) a protein comprising the amino acid sequence represented by SEQ ID NO;
71;
(b) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO;
71 and has GMD activity;
(c) a protein comprising the amino acid sequence represented by SEQ ID NO;
72;
(d) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO;
72 and has Fx activity;
(e) a protein comprising the amino acid sequence represented by SEQ ID NO;
73;
(f) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO;
73 and has GFPP activity;
(g) a protein comprising the amino acid sequence represented by SEQ ID NO;
23;
(h) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO;
23 and has α
-1,6-fucosyltransferase activity;
(i) a protein comprising the amino acid sequence represented by SEQ ID NO;
24;
(j) a protein which comprises an amino acid sequence in which at least one amino acid is deleted, substituted, inserted and/or added in the amino acid sequence represented by SEQ ID NO;
24 and the α
-1,6-fucosyltransferase activity. - View Dependent Claims (58, 61)
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59. A DNA selected from the group consisting of the following (a), (b), (c), (d) and (e):
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(a) a DNA comprising the nucleotide sequence represented by SEQ ID NO;
1;
(b) a DNA comprising the nucleotide sequence represented by SEQ ID NO;
2;
(c) a DNA comprising the nucleotide sequence represented by SEQ ID NO 65;
(d) a DNA comprising the nucleotide sequence represented by SEQ ID NO;
48;
(e) a DNA comprising the nucleotide sequence represented by SEQ ID NO;
51.
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60. A genome DNA selected from the group consisting of the following (a), (b) and (c):
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(a) a genome DNA comprising the nucleotide sequence represented by SEQ ID NO;
3;
(b) a genome DNA comprising the nucleotide sequence represented by SEQ ID NO;
67;
(c) a genome DNA comprising the nucleotide sequence represented by SEQ ID NO;
70.
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Specification