Antisense oligonucleotides with increased RNase sensitivity
First Claim
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1. A method of increasing the effectiveness of an antisense oligonucleotide to cleave a target molecule, comprising:
- providing an antisense oligonucleotide comprising an RNase H activation region; and
adding at least two adjacent universal bases to said oligonucleotide, wherein the addition of said universal bases increases the activity of RNAase H to cleave a target molecule.
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Abstract
Combinatorial libraries comprise first oligonucleotide analogs and second oligonucleotide analogs which are coupled together to form antisense molecules capable of binding target polynucleotides and activating an RNase, and ribozymes capable of cleaving polynucleotides.
76 Citations
39 Claims
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1. A method of increasing the effectiveness of an antisense oligonucleotide to cleave a target molecule, comprising:
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providing an antisense oligonucleotide comprising an RNase H activation region; and
adding at least two adjacent universal bases to said oligonucleotide, wherein the addition of said universal bases increases the activity of RNAase H to cleave a target molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of cleaving a target RNA molecule, comprising:
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providing an antisense oligonucleotide comprising an RNAase H activation region;
adding at least two adjacent universal bases to said oligonucleotide, wherein the addition of said universal bases increases the activity of RNAase H to cleave a target RNA molecule; and
contacting said target RNA molecule with said antisense oligonucleotide in the presence of RNase H. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of making an antisense oligonucleotide that cleaves a target molecule, comprising:
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providing an antisense oligonucleotide comprising an RNAase H activation region; and
adding at least two adjacent universal bases to said oligonucleotide, wherein the addition of said universal bases increases the activity of RNAase H to cleave a target molecule. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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Specification