Forming and modifying dielectrically-engineered microparticles
First Claim
1. An engineered microparticle fabricated to be dielectrically-dispersive and adapted to produce a dielectric response to an applied electric field such that the microparticle is maneuverable by dielectrophoresis.
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Accused Products
Abstract
Engineered microparticles, libraries of microparticles, and methods relating thereto. The microparticles are distinguishable based on differences in dielectric response to an applied electric field. In different embodiments, the dielectric differences may be engineered through, but not limited to, dielectrically dispersive materials, surface charge, and/or fluorescence. Gangliosides may be incorporated with the microparticles to control aggregation. Vesicles including erythrocyte ghosts may be used as a basis for microparticles. The microparticles may utilize a biotin streptavidin system for surface functionalization.
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Citations
60 Claims
- 1. An engineered microparticle fabricated to be dielectrically-dispersive and adapted to produce a dielectric response to an applied electric field such that the microparticle is maneuverable by dielectrophoresis.
- 13. A library of two or more engineered microparticles, each microparticle comprising a dielectrically-dispersive material differing to an extent sufficient to cause a discernible difference in a dielectric response of each microparticle to an applied electric field.
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33. A method for forming a library of two or more engineered microparticles, comprising:
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obtaining a first dielectrically-dispersive material having a first dielectric property;
modifying the first dielectrically-dispersive material to form a second dielectrically-dispersive material having a second dielectric property;
wherein the second dielectric property differs from the first dielectric property to an extent sufficient to cause a discernible difference in a dielectric response to an applied electric field;
forming a first engineered microparticle using the first dielectrically-dispersive material; and
forming a second engineered microparticle using the second dielectrically- dispersive material. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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- 53. An engineered microparticle comprising one or more gangliosides to affect microparticle aggregation.
- 56. A method for controlling the aggregation of microparticles, comprising modulating the surface charge of one or more of the microparticles.
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60. A method for identifying one or more complexes within a sample, the method comprising:
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admixing with the sample a plurality of engineered microparticles, each microparticle comprising streptavidin and having a different dielectric property;
associating the plurality of engineered microparticles with one or more target analytes comprising biotin to form one or more complexes; and
identifying the one or more complexes by distinguishing between the different dielectric properties.
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Specification