Peptide and amine examination method using the same

• US 20030119065A1
• Filed: 10/03/2002
• Published: 06/26/2003
• Est. Priority Date: 12/21/2001
• Status: Active Grant

• Current Assignee: Industrial Technology Research Institute
• Original Assignee: Industrial Technology Research Institute
• Inventors: Lin, Yuh-Jiuan, Guo, Hong-Ru
• Granted Patent: US 7,186,799 B2
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere
• CPC Class Codes: Y10T 436/173845 Amine and quaternary ammonium
• CPC Class Codes: Y10T 436/174614 Tertiary amine
• CPC Class Codes: Y10T 436/175383 Ammonia

Abstract

A peptide for detecting amine, and a method and apparatus for detecting amines using the peptide. The method comprises the application of a sensor to examine a sample, wherein the sensor is coated with the peptide. The result collected from the sensor is compared with a database to determine the amount of amine presenting in the sample.

Claims

1. A peptide, comprising SEQ ID NO:

1, SEQ ID NO;

2, or SEQ ID NO;

3.

2. The peptide as set forth in claim 1, wherein the C- or/and N-terminal of the peptide has attached one or more amino acid groups being alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, or valine.

3. The peptide as set forth in claim 1, wherein the C- or/and N-terminal of the peptide has attached a modifying group being —

COOH, —

NH₂, —

CHO, —

OH, or —

SH group

4. An amine examination method using a peptide having SEQ ID NO:

1, SEQ ID NO;

2, or SEQ ID NO;

3, and the amine is a compound with a NR₃ group, wherein R is an alkyl group or an aryl group;

the method comprising the following steps;

providing a sensory device coated with the peptide;

allowing the sensor to contact with a sample to generate a signal;

processing The result; and

comparing The result with a database.

5. The method as set forth in claim 4, wherein the sensory device is a piezoelectric quartz crystal, surface acoustic wave, electrochemical sensor, fiber optic, surface plasmon resonance, or metal oxide semiconductor.

6. The method as set forth in claim 4, wherein the sensory device is a biochip.

7. The method as set forth in claim 4, wherein the amine is trimethylamine, dimethylamine, monomethylamine, or ammonia.

8. The method as set forth in claim 4, wherein the sample is water, air, animal breath, body fluid of an animal, blood of an animal, urine of an animal, fish or seafood.

9. The method as set forth in claim 4, wherein the sample is human breath.

10. The method as set forth in claim 9, wherein the result is used for diagnosis of disorders accompanied by elevated amine levels detectable in breath.

11. The method as set forth in claim 9, wherein the disorder is kidney disease, uremia, liver disease, or stomach ulcer.

12. An amine examining apparatus comprising a sensory device, a peptide coated on the sensory device, a signal process device coupled to the sensory device, wherein the peptide has SEQ ID NO:

1, SEQ ID NO;

2, or SEQ ID NO;

3.

13. The apparatus as set forth in claim 12, wherein the sensory device is a piezoelectric quartz crystal, surface acoustic wave, electrochemical sensor, fiber optic, surface plasmon resonance, or metal oxide semiconductor.

14. The apparatus as set forth in claim 12, wherein the sensory device is a biochip.