Sensors and methods of detection for proteinase enzymes
First Claim
1. A sensor for detecting proteinase enzymes in a fluid comprising:
- a) a sample reservoir having at least one target antibody and at least one signal element disposed therein, the target antibody bindable to a target proteinase enzyme upon exposure to the fluid to form a target proteinase enzyme target antibody complex; and
b) at least one reaction site in fluid communication with the sample reservoir and having a capture antibody bindable to the proteinase enzyme target antibody complex to form a target proteinase enzyme target antibody complex capture antibody conjugate, thereby indicating the presence of the target proteinase enzyme in the at least one reaction site by causing a detectable or measurable manifestation, wherein the target antibody is stationary within the reaction site.
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Accused Products
Abstract
Sensors for detecting catabolic proteinase enzymes and proenzymes in the fluid of a human or animal and methods for detecting the enzymes and then providing treatment that is specific for the detected enzyme are disclosed. The sensors of the present invention can be used to detect catabolic proteinase enzymes and proenzymes in the fluid of chronic wounds of humans and animals. Upon detection of any proteinase enzyme, the wound can be treated with an inhibiting complex that is specific for the detected enzyme or proenzyme. Enzymes such as matrix metalloproteinases and human neutrophil elastase in the active and proenzyme form can be detected and treatment provided with inhibitors for the detected enzyme.
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Citations
45 Claims
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1. A sensor for detecting proteinase enzymes in a fluid comprising:
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a) a sample reservoir having at least one target antibody and at least one signal element disposed therein, the target antibody bindable to a target proteinase enzyme upon exposure to the fluid to form a target proteinase enzyme target antibody complex; and
b) at least one reaction site in fluid communication with the sample reservoir and having a capture antibody bindable to the proteinase enzyme target antibody complex to form a target proteinase enzyme target antibody complex capture antibody conjugate, thereby indicating the presence of the target proteinase enzyme in the at least one reaction site by causing a detectable or measurable manifestation, wherein the target antibody is stationary within the reaction site. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method for detecting the presence of at least one proteinase enzyme in a fluid of a human or an animal comprising:
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a) providing a sample of the fluid of the human or the animal;
b) exposing the sample to a signal element and at least one target antibody, the at least one target antibody bindable to the at least one proteinase enzyme to form a proteinase enzyme/target antibody complex; and
c) exposing the proteinase enzyme/target antibody complex to form a proteinase enzyme/target antibody complex/capture antibody conjugate, to cause a detectable or measurable manifestation of the signal element, thereby indicating the presence of the at least one proteinase enzyme. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25)
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26. A method for treating chronic wounds in a human or an animal comprising:
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a) providing a sample of the fluid of the human or the animal;
b) exposing the sample to a signal element and at least one target antibody, the at least one target antibody bindable to the at least one proteinase enzyme to form a proteinase enzyme/target antibody complex; and
c) exposing the proteinase enzyme/target antibody complex to form a proteinase enzyme/target antibody complex/capture antibody conjugate, to cause a detectable or measurable manifestation of the signal element, thereby indicating the presence of the at least one proteinase enzyme. d) identifying the at least one proteinase enzyme by determining the presence or absence of a detectable or measurable manifestation of the signal element; and
e) selecting a treatment for the wound that is effective for treating the identified proteinase enzyme. - View Dependent Claims (27, 28, 29, 30)
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31. A sensor for detecting proteinase enzymes in a fluid comprising:
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a) a sample reservoir having at least one target antibody disposed therein, the target antibody bindable to a target proteinase enzyme upon exposure to the fluid to form a target proteinase enzyme target antibody complex; and
b) at least one reaction site in fluid communication with the sample reservoir and having a capture antibody bindable to the proteinase enzyme target antibody complex to form a target proteinase enzyme target antibody complex capture antibody conjugate, thereby indicating the presence of the target proteinase enzyme in the at least one reaction site by causing a detectable or measurable manifestation, wherein the target antibody is stationary within the reaction site. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38)
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39. A method for detecting the presence of at least one proteinase enzyme in a fluid of a human or an animal comprising:
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a) providing a sample of the fluid of the human or the animal;
b) exposing the sample to at least one target antibody, the at least one target antibody bindable to the at least one proteinase enzyme to form a proteinase enzyme/target antibody complex; and
c) exposing the proteinase enzyme/target antibody complex to form a proteinase enzyme/target antibody complex/capture antibody conjugate, to cause a detectable or measurable manifestation, thereby indicating the presence of the at least one proteinase enzyme. - View Dependent Claims (40, 41, 42)
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43. A method for treating chronic wounds in a human or an animal comprising:
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a) providing a sample of the fluid of the human or the animal;
b) exposing the sample to at least one target antibody, the at least one target antibody bindable to the at least one proteinase enzyme to form a proteinase enzyme/target antibody complex; and
c) exposing the proteinase enzyme/target antibody complex to form a proteinase enzyme/target antibody complex/capture antibody conjugate, to cause a detectable or measurable manifestation, thereby indicating the presence of the at least one proteinase enzyme. d) identifying the at least one proteinase enzyme by determining the presence or absence of a detectable or measurable manifestation; and
e) selecting a treatment for the wound that is effective for treating the identified proteinase enzyme. - View Dependent Claims (44, 45)
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Specification