Method for producing target substance

• US 20030119155A1
• Filed: 09/05/2002
• Published: 06/26/2003
• Est. Priority Date: 09/06/2001
• Status: Active Grant

• Current Assignee: Ajinomoto Co., Inc.
• Original Assignee: Ajinomoto Co., Inc.
• Inventors: Takeshita, Ryo, Yasueda, Hisashi
• Granted Patent: US 7,163,810 B2
• Time in Patent Office: Days
• US Class Current: 435/115
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12P 13/08 Lysine; Diaminopimelic acid; Threonine; Valine

Abstract

In a method for producing a target substance by using a microorganism comprising culturing a microorganism having an ability to produce the target substance in a medium to produce and accumulate the target substance in the medium or cells of the microorganism and collecting the target substance from the medium or the cells of the microorganism, there are used, as the microorganism, a microorganism to which a methanol dehydrogenase gene is introduced, of which activities of hexulose phosphate synthase and phosphohexuloisomerase are enhanced and which is modified so that an ability to utilize methanol should be imparted or enhanced, and there is used a medium containing methanol as a carbon source.

Claims

1. A method for producing a target substance by using a microorganism comprising culturing a microorganism having an ability to produce the target substance in a medium to produce and accumulate the target substance in the medium or cells of the microorganism and collecting the target substance from the medium or the cells of the microorganism, wherein the microorganism is a microorganism to which a methanol dehydrogenase gene is introduced and which is modified so that activities of hexulose phosphate synthase and phosphohexuloisomerase should be enhanced and an ability to utilize methanol should be imparted or enhanced, and the medium contains methanol as a carbon source.

2. The method according to claim 1, wherein the microorganism is a microorganism to which a gene coding for a methanol dehydrogenase activator is further introduced.

3. The method according to claim 1 or 2, wherein the target substance is an L-amino acid.

4. The method according to claim 3, wherein the L-amino acid is L-lysine.

5. The method according to claim 3, wherein the microorganism is a bacterium belonging to the genus Bacillus.

6. A microorganism to which a methanol dehydrogenase gene is introduced, wherein the microorganism is modified so that hexulose phosphate synthase and phosphohexuloisomerase should be enhanced and an ability to utilize methanol should be imparted or enhanced.

7. The microorganism according to claim 6, to which a gene coding for a methanol dehydrogenase activator is further introduced.

8. The microorganism according to claim 6 or 7, which is a Gram positive bacterium.

9. The microorganism according to claim 8, which is a bacterium belonging to the genus Bacillus.

10. The microorganism according to claim 9, which is Bacillus subtilis.