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Internal calibration system for flow-through assays

  • US 20030119204A1
  • Filed: 04/25/2002
  • Published: 06/26/2003
  • Est. Priority Date: 12/24/2001
  • Status: Abandoned Application
First Claim
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1. A flow-through assay for detecting the presence or quantity of an analyte residing in a test sample, said flow-through assay comprising a porous membrane, wherein said porous membrane is in fluid communication with a probe conjugate that contains a specific binding member and a detectable probe, said porous membrane defining:

  • a detection zone that contains a capture reagent that is capable of binding to the analyte or the probe conjugate, wherein said detection zone is capable of generating a detection signal that represents the presence or absence of the analyte;

    a calibration zone that contains a binder configured to bind with said probe conjugate, said calibration zone including;

    i) a first calibration region containing a first predetermined amount of said binder, said first calibration region being capable of generating a first calibration signal;

    ii) a second calibration region containing a second predetermined amount of said binder that is greater than said first predetermined amount of said binder, said second calibration region being capable of generating a second calibration signal, said second calibration signal having a greater intensity than said first calibration signal; and

    wherein the relative amount of the analyte within the test sample is determined by comparing said detection signal to said first calibration signal and said second calibration signal.

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