Electrochemical immunosensor and kit and method for detecting biochemical anylyte using the sensor
First Claim
1. An electrochemical immunosensor comprising:
- a substrate;
an electrode or an electrode array formed on the substrate; and
a biological sensor layer formed on the electrode or the electrode array and including a polymeric dendrimer monolayer with an antigen or a ligand residue immobilized on the surface thereof.
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Abstract
An electrochemical immunosensor including a biological sensor layer with an antigen or a ligand residue immobilized thereon, and a biochemical analyte detection kit and method for electrochemically signaling a biological reaction occurring in the biological sensor layer are provided. The electrochemical immunosensor includes a substrate, an electrode or an electrode array formed on the substrate, and a biological sensor layer formed on the electrode or the electrode array and including a polymeric dendrimer monolayer with an antigen or a ligand residue immobilized on the surface thereof. The biological sensor layer further includes an adhesive layer for biomolecular immobilization between the electrode and the polymeric dendrimer monolayer.
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Citations
20 Claims
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1. An electrochemical immunosensor comprising:
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a substrate;
an electrode or an electrode array formed on the substrate; and
a biological sensor layer formed on the electrode or the electrode array and including a polymeric dendrimer monolayer with an antigen or a ligand residue immobilized on the surface thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 17, 18, 19, 20)
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10. A biochemical analyte detection kit comprising:
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an electrochemical immunosensor having a biological sensor layer including a self-assembled monolayer formed on an electrode and a polymeric dendrimer monomer with an antigen or a ligand residue immobilized on the surface thereof;
a buffer solution as a dilution of an antibody or a receptor capable of specifically binding to the antigen or the ligand residue in the polymeric dendrimer monolayer, respectively;
a precipitation substrate; and
a labeled catalytic enzyme capable of binding to an antibody or a receptor to discriminate whether a specific interaction between the antigen and the antibody or between the ligand residue and the receptor has occurred and inducing precipitation from the precipitation substrate. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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Specification