Method of treating and preventing infectious diesases
First Claim
1. A method for reducing levels of a lipid-containing infectious organism in a fluid comprising:
- contacting the fluid containing the lipid-containing infectious organism with a first organic solvent capable of extracting lipid from the lipid-containing infectious organism;
mixing the fluid and the first solvent;
permitting organic and aqueous phases to separate; and
collecting the aqueous phase containing the infectious organism with reduced lipid content.
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Abstract
The present invention relates to a method for reducing the occurrence and severity of infectious diseases, especially infectious diseases in which lipid-containing infectious organisms are found in biological fluids, such as blood. The present invention employs solvents useful for extracting lipids from the lipid-containing infectious organism, thereby reducing the infectivity of the infectious organism. The present invention also provides a vaccine composition, comprising a lipid-containing infectious organism, treated with solvents to reduce the lipid content of the infectious organism, combined with a pharmaceutically acceptable carrier. The vaccine composition is administered to an animal or a human to provide protection against the lipid-containing infectious organism.
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Citations
28 Claims
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1. A method for reducing levels of a lipid-containing infectious organism in a fluid comprising:
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contacting the fluid containing the lipid-containing infectious organism with a first organic solvent capable of extracting lipid from the lipid-containing infectious organism;
mixing the fluid and the first solvent;
permitting organic and aqueous phases to separate; and
collecting the aqueous phase containing the infectious organism with reduced lipid content. - View Dependent Claims (2, 12, 13, 14, 15, 16, 17, 18)
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3. A method for reducing levels of a lipid-containing infectious organism in an animal or a human comprising:
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obtaining a fluid containing the lipid-containing infectious organism from the animal or the human;
contacting the fluid containing the lipid-containing infectious organism with a first organic solvent capable of extracting lipid from the lipid-containing infectious organism;
mixing the fluid and the first organic solvent;
permitting organic and aqueous phases to separate;
collecting the aqueous phase containing the infectious organism with reduced lipid content; and
introducing the aqueous phase containing the infectious organism with reduced lipid content into the animal or the human. - View Dependent Claims (4)
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5. A method for reducing levels of a lipid-containing infectious organism in animal or human plasma comprising:
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removing blood containing the lipid-containing infectious organism from the animal or the human;
obtaining plasma from the blood, the plasma containing the lipid-containing infectious organism;
contacting the plasma containing the lipid-containing infectious organism with a first organic solvent capable of extracting lipid from the lipid-containing infectious organism;
mixing the plasma and the first organic solvent;
permitting organic and aqueous phases to separate;
collecting the aqueous phase containing the infectious organism with reduced lipid content; and
introducing the aqueous phase containing the infectious organism with reduced lipid content into the animal or the human. - View Dependent Claims (6, 7)
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8. A method for making a vaccine comprising:
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contacting a lipid-containing infectious organism in a fluid with a first organic solvent capable of extracting lipid from the lipid-containing infectious organism;
mixing the fluid and the first organic solvent for a time sufficient to extract lipid from the lipid-containing infectious organism;
permitting organic and aqueous phases to separate; and
collecting the aqueous phase containing the infectious organism with reduced lipid content. - View Dependent Claims (9, 10, 11, 19)
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- 20. A vaccine composition, comprising a substantially delipidated infectious organism and a pharmaceutically acceptable carrier.
- 26. Use of an organic solvent mixture comprising diisopropyl ether and butanol for the preparation of a solvent system for treating an animal or a human infected by a lipid-containing infectious organism by obtaining a fluid from the animal or the human, contacting the fluid with the solvent system for a time sufficient to reduce active levels of the lipid-containing infectious organism in the fluid, separating the fluid from the solvent system, and reintroducing the fluid into the animal or the human, whereby dissolved lipids are separated with and remain in the solvent system.
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28. Use of an organic solvent mixture comprising diisopropyl ether and butanol for the preparation of a solvent system for treating an animal or a human infected by a lipid-containing infectious organism by obtaining blood from the animal or the human, separating blood cells to obtain plasma, contacting the plasma with the solvent system for a time sufficient to reduce active levels of the lipid-containing infectious organism in the plasma, separating the plasma from the solvent system, and reintroducing the plasma into the animal or the human, whereby dissolved lipids are separated with and remain in the solvent system.
Specification