Polyfunctional base sequence and artificial gene containing the same
First Claim
1. A multifunctional base sequence wherein the base sequence has two or more functions in different reading frames of the base sequence.
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Abstract
The present invention provides: a microgene comprising a multifunctional base sequence having two or more functions in different reading frames of the base sequence which is required for creating industrially useful artificial proteins that do not exist in nature; an artificial gene obtained by polymerizing the microgenes; and an artificial protein which is a translation product of the artificial gene.
A microgene is produced by selecting a base sequence by the computational science approach from among all combinations of base sequences encoding an amino acid sequence having a given function where the selected base sequence has a biological function same as or different from the given function in a reading frame different from that of the amino acid sequence having the given function, and is devoid of termination codons in all three reading frames; the microgene thus obtained is then polymerized in such a manner as plural reading frames emerge to produce an artificial gene; and the artificial protein as a translation product of the artificial gene is obtained.
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Citations
51 Claims
- 1. A multifunctional base sequence wherein the base sequence has two or more functions in different reading frames of the base sequence.
- 14. A method of producing a multifunctional base sequence having two or more functions wherein a base sequence is selected, from among all the combinations of base sequences encoding an amino acid sequence having a given function, which has a function same as or different from the given function in a reading frame different from that of the amino acid sequence having the given function.
Specification