Proteomic analysis of tumors for development of consultative report of therapeutic options

• US 20030124130A1
• Filed: 12/19/2002
• Published: 07/03/2003
• Est. Priority Date: 01/02/2002
• Status: Abandoned Application

• Current Assignee: Geisinger Medical Center (Geisinger Health System)
• Original Assignee: Geisinger Medical Center (Geisinger Health System)
• Inventors: Brown, Robert E.
• Application Number:
  US10/325,793
• Publication Number:
  US 20030124130A1
• Time in Patent Office: Days
• US Class Current: 424/155.1
• CPC Class Codes: G01N 33/57484 involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
• CPC Class Codes: G01N 33/6803 General methods of protein analysis not limited to specific proteins or families of proteins

Abstract

The present invention provides methods of identifying potential therapeutic options for treatment of a tumor, and a consultative report providing the same.

Claims

1. A method of identifying at least one potential therapeutic option for treatment of a tumor comprising the steps of:

correlating a cluster of protein expression for a proteomic profile of said tumor to at least one molecular pathway of said tumor; and

identifying at least one potential therapeutic option based upon said molecular pathway identified.

2. The method of claim 1 further comprising the step of generating a proteomic profile of said tumor prior to said correlating a cluster of protein expression.

3. The method of claim 1 wherein said proteomic profile of said tumor comprises an immunohistochemical or immunofluorescent analysis of said tumor.

4. The method of claim 3 wherein said immunohistochemical or immunofluorescent analysis comprises using a panel of at least five antibodies directed to different molecular targets in said tumor.

5. The method of claim 3 wherein said immunohistochemical or immunofluorescent analysis comprises using a panel of at least ten antibodies directed to different biological targets in said tumor.

6. The method of claim 3 wherein said immunohistochemical or immunofluorescent analysis comprises using a panel of at least fifteen antibodies directed to different biological targets in said tumor.

7. The method of claim 4 wherein said immunohistochemical or immunofluorescent analysis further comprises determining the cellular location of a biological target in a cell of said tumor.

8. The method of claim 4 wherein said molecular targets are selected from the group consisting of Her-2/neu, Ki-67, p53, Cyclin D1, c-Jun, ER, PR, gp130, IL-6, IL-11, EGFR, TGF-α

, farnesyl transferase, p21^ras, the latency-associated peptide of TGF-β

₁, TGF-β

RII, bcl-2, and COX-2.

9. The method of claim 1 wherein said tumor is selected from the group consisting of oligodendroglioma, ependymoma, meningioma, lymphoma, Ewing'"'"'s sarcoma, chondrosarcoma, osteosarcoma, rhabdomyosarcoma, Schwannoma, medulloblastoma, breast, adrenal, pancreatic, parathyroid, pituitary, thyroid, anal, colorectal, esophageal, gall bladder, gastric, hepatoma, small intestine, cervical, endometrial, uterine, fallopian tube, ovarian, vaginal, vulvar, laryngeal, oropharyngeal, acute lymphocytic leukemia, acute myelogenous leukemia, chronic lymphocytic leukemia, chronic myogenous leukemia, hairy cell leukemia, mesothelioma, non small-cell lung carcinoma, small cell-lung carcinoma, AIDS-related lymphoma, cutaneous T-cell lymphoma, Hodgkin'"'"'s disease, non-Hodgkins, myeloma, penile, prostrate, melanoma, Kaposi'"'"'s sarcoma, testicular, bladder, kidney, and urethral tumors.

10. The method of claim 1 wherein said identifying a potential therapeutic option comprises identifying a pharmaceutical agent that is targeted to a protein involved in said molecular pathway identified.

11. A method of identifying at least one potential therapeutic option for treatment of a tumor comprising the steps of:

correlating a cluster of protein expression for a proteomic profile comprising an immunohistochemical or immunofluorescent analysis of said tumor to at least one physiological pathway of said tumor, wherein said immunohistochemical or immunofluorescent analysis comprises using a panel of at least five antibodies directed to different biological targets selected from the group consisting of Her-2/neu, Ki-67, p53, Cyclin D1, c-Jun, ER, PR, gp130, IL-6, IL-11, EGFR, TGF-α

, farnesyl transferase, p21^ras, the latency-associated peptide of TGF-β

₁, TGF-β

RII, bcl-2, and COX-2 in said tumor; and

identifying at least one potential therapeutic option based upon said physiological pathway identified.

12. A method of generating a consultative report that identifies at least one potential therapeutic option for treatment of a tumor from a patient comprising the steps of:

listing at least one molecular pathway identified as being a part of the pathology for said tumor from said patient; and

listing at least one pharmaceutical agent that is directed to a biological target involved in said molecular pathway identified.

13. The method of claim 12 wherein said tumor is selected from the group consisting of oligodendroglioma, ependymoma, meningioma, lymphoma, Ewing'"'"'s sarcoma, chondrosarcoma, osteosarcoma, rhabdomyosarcoma, Schwannoma, medulloblastoma, breast, adrenal, pancreatic, parathyroid, pituitary, thyroid, anal, colorectal, esophageal, gall bladder, gastric, hepatoma, small intestine, cervical, endometrial, uterine, fallopian tube, ovarian, vaginal, vulvar, laryngeal, oropharyngeal, acute lymphocytic leukemia, acute myelogenous leukemia, chronic lymphocytic leukemia, chronic myogenous leukemia, hairy cell leukemia, mesothelioma, non small-cell lung carcinoma, small cell-lung carcinoma, AIDS-related lymphoma, cutaneous T-cell lymphoma, Hodgkin'"'"'s disease, non-Hodgkin'"'"'s lymphoma, myeloma, penile, prostrate, melanoma, Kaposi'"'"'s sarcoma, testicular, bladder, kidney, and urethral tumors.

14. The method of claim 12 further comprising the step of listing a plurality of molecular pathways potentially involved in said pathology for said tumor.

15. The method of claim 12 further comprising the step of listing at least one biological target involved in said molecular pathway for said tumor.

16. The method of claim 15 wherein said at least one biological target is selected from the group consisting of Her-2/neu, Ki-67, p53, Cyclin D1, c-Jun, ER, PR, gp130, IL-6, IL-11, EGFR, TGF-α

, farnesyl transferase, p21^ras, the latency-associated peptide of TGF-β

₁, TGF-β

RII, bcl-2, and COX-2.

17. The method of claim 12 further comprising the step of listing a plurality of biological targets potentially involved in said molecular pathway for said tumor.

18. The method of claim 14 further comprising the step of listing a score for at least one of said plurality of molecular pathways potentially involved in said pathology for said tumor.

19. The method of claim 17 further comprising the step of listing a score for at least one of said plurality of biological targets potentially involved in said molecular pathway for said tumor.

20. The method of claim 18 wherein said score is a value corresponding to the level of involvement of a molecular pathway in said pathology for said tumor.

21. The method of claim 19 wherein said score is a value corresponding to the level of involvement of said at least one of said plurality of biological targets in said molecular pathway for said tumor.

22. The method of claim 20 wherein- said score is a measure of a proteomic profile of said tumor.

23. The method of claim 21 wherein said score is a measure of a proteomic profile of said tumor.

24. The method of claim 12 further comprising the step of listing a narrative interpretation.

25. The method of claim 24 wherein said narrative interpretation comprises at least one of literature citation or summary regarding said tumor, literature citation or summary regarding said at least one molecular pathway identified as being a pathology for said tumor from said patient, literature citation or summary regarding said at least one pharmaceutical agent that is directed to a biological target involved in said molecular pathway identified, literature citation or summary regarding side-effects of listed pharmaceutical agents, literature citation or summary regarding said biological target, summary of expected prognosis, and literature citation or summary regarding precedent for treatment with said at least one pharmaceutical agent.

26. The method of claim 12 further comprising the step of listing the name of said patient.

27. The method of claim 12 further comprising the step of listing the name of at least one physician.

28. The method of claim 12 further comprising the step of listing an interpretation of said score for said molecular pathway, biological target, or both.

29. The method of claim 12 further comprising the step of listing the cellular compartment in which said biological target was detected.