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Methods for mapping the chromosomal loci of genes expressed by a cell

  • US 20030124542A1
  • Filed: 02/08/2002
  • Published: 07/03/2003
  • Est. Priority Date: 12/28/2001
  • Status: Abandoned Application
First Claim
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1. An array-based method for identifying a chromosomal loci of a gene expressed by a cell, the method comprising:

  • (a) providing an array comprising a plurality of cloned genomic nucleic acid segments, wherein each genomic nucleic acid segment is immobilized to a discrete and known spot on a substrate surface to form an array and the cloned genomic nucleic acid segments comprise a substantially complete genome or a known subset of a genome;

    (b) providing a sample comprising a nucleic acid sequence comprising a detectable label, wherein the nucleic acid sequence comprises the same sequence or a sequence complementary to a sequence of a transcript expressed by a cell;

    (c) contacting the sample of step (b) with the array of step (a) under conditions wherein the labeled nucleic acid can specifically hybridize to the genomic nucleic acid segments immobilized on the array; and

    , (d) identifying to which discrete and known spots on the substrate surface are specifically hybridized to a labeled nucleic acid segment, wherein the identification is made by detecting a specifically hybridized nucleic acid sequence, thereby identifying a chromosomal loci of a gene expressed by a cell.

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