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Microfluidic device and surface decoration process for solid phase affinity binding assays

  • US 20030124623A1
  • Filed: 12/05/2002
  • Published: 07/03/2003
  • Est. Priority Date: 12/05/2001
  • Status: Active Grant
First Claim
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1. A microfluidic device for detecting the presence of an analyte in a fluid sample comprising:

  • a) a microfluidic channel comprising a bottom wall, two side walls and a top wall and having an upstream end and a downstream end;

    b) a fluid inlet in fluidic connection with said microfluidic channel;

    c) a storage area on one of said walls of said microfluidic channel downstream of said fluid inlet;

    d) at least one solid reagent plug fixed in said storage area, said plug comprising a matrix material and a reagent having an affinity for binding to said analyte; and

    e) a detection area within said microfluidic channel downstream of said fluid inlet and said storage area wherein said reagent binds to a binding wall of the microfluidic channel.

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