Collection systems for cytometer sorting of sperm
First Claim
Patent Images
1. An improved flow cytometer system for isolating desired cells comprising:
- a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells which contains about 2.9% sodium citrate;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed.
9 Assignments
0 Petitions
Accused Products
Abstract
Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.
-
Citations
123 Claims
-
1. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells which contains about 2.9% sodium citrate;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed. - View Dependent Claims (2, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
3. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells which contains a hepes buffered medium;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed. - View Dependent Claims (4)
-
-
5. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed and which comprises a citrate collector fluid containing about six percent egg yolk. - View Dependent Claims (6, 7)
-
-
8. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed and which comprises a test tube having the physical characteristics of a stream-matched container. - View Dependent Claims (9, 13)
-
-
22. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a chemically coordinated sheath fluid source which creates a sheath fluid environment for said cells which is selected to be coordinated with both a pre-sort and a post-sort cell fluid environment;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 42, 43, 44, 45, 47, 48, 49, 50, 51, 52, 53)
-
-
41. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells which comprises a solution containing about 2.9% sodium citrate;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed.
-
-
46. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells which comprises a solution containing a hepes buffered medium;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed.
-
-
54. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies bovine sperm cells to be analyzed by the flow cytometer;
b. a chemically coordinated sheath fluid source which creates a sheath fluid environment for said cells which is contains about 2.9% sodium citrate;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic;
g. a collector into which cells having a desired characteristic which contains a citrate collection fluid comprising about six percent egg yolk and which is used to provide a dosage of less than about five hundred thousand sperm;
and wherein said nozzle, oscillator, cell sensing system, and sorter discrimination system are part of a flow cytometer system which sorts said cells to be analyzed at a rate of at least about five hundred sorts per second and operates at a pressure of at least about fifty pounds per square inch.
-
-
55. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies equine sperm cells to be analyzed by the flow cytometer;
b. a chemically coordinated sheath fluid source which creates a sheath fluid environment for said cells which is contains a hepes buffered medium;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic;
g. a collector into which cells having a desired characteristic which contains a collection fluid comprising a hepes buffered medium and which is used to provide a dosage of less than about ten million sperm;
and wherein said nozzle, oscillator, cell sensing system, and sorter discrimination system are part of a flow cytometer system which sorts said cells to be analyzed at a rate of at least about five hundred sorts per second and operates at a pressure of at least about fifty pounds per square inch.
-
-
56. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a means for minimizing the changes between a sheath fluid environment for said cells and both a pre-sort and a post-sort cell fluid environment;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed. - View Dependent Claims (57, 58)
-
-
59. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector into which cells having a desired characteristic are placed which comprises a chemically coordinated collector fluid sheath fluid source which creates a collector fluid environment for said cells which is selected to be coordinated with a prior cell fluid environment. - View Dependent Claims (60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 81, 82, 83, 84, 85, 86, 87)
-
-
74. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector which comprises a cushioning element. - View Dependent Claims (75, 76, 77, 78, 79, 80, 88, 89, 90)
-
-
91. An improved flow cytometer system for isolating desired cells comprising:
-
a. a cell source which supplies cells to be analyzed by the flow cytometer;
b. a sheath fluid source which creates a sheath fluid environment for said cells;
c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
e. a cell sensing system which responds to said cells;
f. a sorter discrimination system which acts to sort cells having a desired characteristic; and
g. a collector which comprises a means for avoiding impact between said cells and said collector.
-
-
92. A method of sorting cells comprising the steps of:
-
a. establishing a cell source which supplies cells to be sorted;
b. chemically coordinating a sheath fluid to create a sheath fluid environment for said cells which is coordinated with both a pre-sort and a post-sort cell fluid environment;
c. sensing a property of said cells;
d. discriminating between cells having a desired characteristic; and
e. collecting cells having the desired characteristic. - View Dependent Claims (93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 116, 117, 118, 119, 120, 121, 122, 123)
-
-
105. A method of sorting cells comprising the steps of:
-
a. establishing a cell source which supplies cells to be sorted;
b. establishing a sheath fluid to create a sheath fluid environment for said cells;
c. sensing a property of said cells;
d. discriminating between cells having a desired characteristic;
e. collecting cells having the desired characteristic in a collector fluid; and
f. chemically coordinating said collector fluid to create an ending collector fluid environment for said cells which is coordinated with a pre-sort fluid environment. - View Dependent Claims (106, 107, 108, 109)
-
-
110. A method of sorting cells comprising the steps of:
-
a. establishing a cell source which supplies cells to be sorted;
b. establishing a sheath fluid to create a sheath fluid environment for said cells;
c. sensing a property of said cells;
d. discriminating between cells having a desired characteristic;
e. collecting cells having the desired characteristic comprising the step of cushioning said cells from impact with a collection container. - View Dependent Claims (111, 112, 113, 114, 115)
-
Specification