Mesoscale detection structures
First Claim
1. A device for detecting the presence of an analyte in a fluid sample, the device comprising a solid substrate microfabricated to define:
- a sample inlet port; and
a mesoscale flow system comprising;
a sample flow channel extending from said inlet port; and
an analyte detection region in fluid communication with said flow channel comprising a binding moiety for specifically binding said analyte, said detection region having a mesoscale dimension.
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Abstract
Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.
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Citations
42 Claims
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1. A device for detecting the presence of an analyte in a fluid sample, the device comprising a solid substrate microfabricated to define:
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a sample inlet port; and
a mesoscale flow system comprising;
a sample flow channel extending from said inlet port; and
an analyte detection region in fluid communication with said flow channel comprising a binding moiety for specifically binding said analyte, said detection region having a mesoscale dimension. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 41)
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27. A method for detecting the presence of an analyte in a fluid sample, the method comprising the steps of:
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(i) providing a device comprising a solid substrate microfabricated to define;
a sample inlet port; and
a mesoscale flow system comprising;
a sample flow channel extending from said inlet port; and
an analyte detection region in fluid communication with said flow channel comprising a binding moiety for specifically binding said analyte, said detection region having a mesoscale dimension;
(ii) delivering said sample to said inlet port and through said flow system to said detection region; and
(iii) detecting the binding of said analyte to said binding moiety in said detection region. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 42)
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38. An appliance for use with an analytical device including a solid substrate microfabricated to define at least one inlet port and a mesoscale flow system, extending from an inlet port on said substrate, the appliance comprising:
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means for holding said substrate;
fluid input means interfitting with an inlet port on said substrate; and
pump means for passing fluid through the flow system of said substrate when is held in said holding means. - View Dependent Claims (39, 40)
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Specification