Binding protein as biosensors
First Claim
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1. A biosensor comprising:
- a) at least one mutated binding protein and at least one thiol group attached thereto; and
b) at least one sensor surface wherein said mutated binding protein is coupled through said thiol group to said surface;
wherein said at least one sensor surface provides a detectable signal resulting from a change in refractive index when said mutated binding protein binds to analyte.
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Abstract
The invention is directed to compositions of mutated binding proteins containing thiol groups for coupling to sensor surfaces, analyte biosensor devices derived there from, and methods of their use as analyte biosensors both in vitro and in vivo.
25 Citations
28 Claims
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1. A biosensor comprising:
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a) at least one mutated binding protein and at least one thiol group attached thereto; and
b) at least one sensor surface wherein said mutated binding protein is coupled through said thiol group to said surface;
wherein said at least one sensor surface provides a detectable signal resulting from a change in refractive index when said mutated binding protein binds to analyte. - View Dependent Claims (3, 6)
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- 2. The biosensor of claim 2 wherein said mutated binding protein is selected from glucose/galactose binding proteins.
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9. The biosensor of 8 wherein said mutated glucose binding protein further includes a histidine tag.
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11. The biosensor of 10 wherein said mutated glucose binding protein further includes a histidine tag.
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13. The biosensor of 12 wherein said mutated glucose binding protein further includes a histidine tag.
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14. A method for analyte detection comprising:
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a) providing at least one mutated binding protein and at least one thiol group attached thereto;
b) at least one sensor surface wherein said mutated binding protein is coupled through said thiol group to said surface;
c) exposing said mutated binding protein to biological solutions containing varying analyte concentrations;
d) detecting a detectable and reversible signal resulting from a change in refractive index;
wherein said detectable and reversible signal results from a change in refractive index upon binding corresponding to said varying analyte concentrations. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification