Encapsulation of discrete quanta of fluorescent particles
First Claim
1. A composition comprising at least two types of discrete fluorescent materials, each capable of emitting a distinct fluorescent light, said materials encapsulated by a shell barrier.
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Abstract
The present invention provides novel encapsulation compositions and methods. In particular, the invention relates to fluorescent capsule compositions, which consists of a layer of a polymer shell enclosing one or more fluorescent materials such as fluorescent microspheres and which are capable of emitting at least two distinct fluorescent signals. Also provided are methods for their preparation. The compositions and methods of this invention are useful in a variety of applications, including preparation of multiplexed arrays for industrial, chemical, immunological, and genetic manipulation and analysis especially as related but not limited to flow cytometry.
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Citations
15 Claims
- 1. A composition comprising at least two types of discrete fluorescent materials, each capable of emitting a distinct fluorescent light, said materials encapsulated by a shell barrier.
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7. A fluorescence encapsulation device comprising an inner zone delimited by a capsule shell that inhibits the leakage of fluorescence emitting materials, wherein the capsule shell comprises a hydrophilic or a hydrophobic polymer material, wherein the inner zone comprises two or more fluorescent light emitting materials each capable of emitting at least one distinct fluorescent signal.
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8. A method of making a capsule, which emits at least two distinct fluorescent signals comprising the steps of:
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a) mixing in a matrix material at least two fluorescent materials at a desired ratio;
b) forming a droplet out of mixture obtained at step (a), and;
c) encapsulating formed droplet by a capsule material.
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9. A process for encapsulating two or more fluorescent materials each capable of emitting at least one distinct fluorescent signal in a shell comprising:
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a) dispersing or dissolving said fluorescent materials in a matrix-forming material, b) adding a shell material or precursor thereof to stirred fluorescent materials in the matrix-forming material, and c) polymerizing the shell material or its precursor to the polymerized shell which encapsulates the fluorescent materials.
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10. A process for encapsulating fluorescent materials in a shell comprising:
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a) preparing an encapsulation composition by dispersing or dissolving said fluorescent materials in a matrix-forming material, b) emulsifying the encapsulation composition in a solution of gelatin, c) mixing at desired rate the obtained solution to allow said encapsulation composition to break up into droplets of desired size, d) adding a solution of gum arabic, e) thermally softening the obtained solution while adding warm water, f) bringing the pH of the solution to about 4.0 and 4.4, g) cooling the solution and adding a cross-linking agent to form the capsule shell, h) bringing the pH to about 9, and;
i) curing the capsule shell by raising the temperature to about 50°
C.
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- 11. A composition comprising (i) two or more substances, each substance capable of emitting a distinctive fluorescent signal, (ii) a shell barrier encapsulating said two or more substances.
Specification