Polymorphism detection
First Claim
1. An array of oligonucleotide probes for detecting a previously identified polymorphism in a target nucleic acid sequence, said array comprising at least one detection block of probes selected for detection of the previously identified polymorphism said detection block including first and second groups of probes that are complementary to said target nucleic acid sequence having first and second variants of said polymorphism, respectively, and further comprising third and fourth groups of probes, said third and fourth groups of probes having a sequence identical to said first and second groups of probes, respectively, except that said third and fourth groups of probes include all possible monosubstitutions of positions in said sequence that are within n bases of a base in said sequence that is complementary to said polymorphism, wherein n is from 1 to 5.
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Abstract
The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an individual. The probes are typically arranged in detection blocks, each block being capable of discriminating the three genotypes for a given marker, e.g., the heterozygote or either of the two homozygotes. The method allows for rapid, automatable analysis of genetic linkage to even complex polygenic traits.
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Citations
6 Claims
- 1. An array of oligonucleotide probes for detecting a previously identified polymorphism in a target nucleic acid sequence, said array comprising at least one detection block of probes selected for detection of the previously identified polymorphism said detection block including first and second groups of probes that are complementary to said target nucleic acid sequence having first and second variants of said polymorphism, respectively, and further comprising third and fourth groups of probes, said third and fourth groups of probes having a sequence identical to said first and second groups of probes, respectively, except that said third and fourth groups of probes include all possible monosubstitutions of positions in said sequence that are within n bases of a base in said sequence that is complementary to said polymorphism, wherein n is from 1 to 5.
Specification