Enzymes for the detection of nucleic acid sequences
First Claim
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1. A composition comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 279 and the complement of SEQ ID NO;
279.
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Abstract
The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
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Citations
6 Claims
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1. A composition comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 279 and the complement of SEQ ID NO;
279. - View Dependent Claims (2, 3)
- 279 and the complement of SEQ ID NO;
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4. A composition comprising a polypeptide encoded by a nucleic acid selected from the group consisting of SEQ ID NO:
- 279 and sequences that hybridize to the complement of SEQ ID NO;
279 under stringent conditions. - View Dependent Claims (5)
- 279 and sequences that hybridize to the complement of SEQ ID NO;
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6. A method for producing an altered enzyme with improved functionality in a nucleic acid cleavage assay comprising:
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a) providing;
i) a polypeptide comprising SEQ ID NO;
280;
ii) a nucleic acid encoding a polypeptide of SEQ ID NO;
280; and
iii) a nucleic acid test substrate;
b) introducing one or more heterologous domains into said nucleic acid to produce an altered nucleic acid encoding an altered enzyme;
c) contacting said altered enzyme and said polypeptide comprising SEQ ID NO;
280 with said nucleic acid test substrate to produce cleavage products; and
d) comparing cleavage product contacted with said altered enzyme to cleavage products contacted with said polypeptide comprising SEQ ID NO;
280.
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Specification