Compositions and methods for performing biological reactions

• US 20030134410A1
• Filed: 11/14/2002
• Published: 07/17/2003
• Est. Priority Date: 11/14/2002
• Status: Abandoned Application

• Current Assignee: GE Healthcare Bio-Sciences AB (General Electric Company)
• Original Assignee: Amersham Biosciences (General Electric Company)
• Inventors: Silva, Robin M., Hawkins, George W.
• Application Number:
  US10/149,660
• Publication Number:
  US 20030134410A1
• Time in Patent Office: Days
• US Class Current: 435/287.2
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

The present invention relates to an apparatus for performing biological reactions. Specifically, the invention relates to an apparatus for performing nucleic acid hybridization reactions on a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon, using substrates with flexible covers.

Claims

1. An apparatus for performing biological reactions comprising:

a) a substrate having a first surface and a second surface;

b) an array of biomolecules positioned on said first surface;

c) a flexible layer affixed to said first surface by an adhesive layer to create a reaction volume; and

d) a port extended from said second surface to said reaction volume of said first surface.

3. An apparatus according to claim 1 or 2 wherein said substrate is glass, silicon, ceramic or plastic.

4. An apparatus according to claim 1, 2 or 3 wherein said biomolecules are nucleic acids.

5. An apparatus according to claim 1, 2, 3 or 4 wherein reaction volume comprises a water soluble compound.

6. An apparatus according to claim 1, 2, 3, 4 or 5 wherein said biomolecules are attached to said substrate using a gel pad.

7. An apparatus according to claim 1, 2, 3, 4, 5 or 6 further comprising a scanner.

2. An apparatus for performing biological reactions comprising:

a) a substrate having a first surface;

b) an array of biomolecules positioned on said first surface; and

c) a flexible layer affixed to said first surface by an adhesive layer to create a reaction volume, comprising at least a first port into said reaction volume.