Islet cells from human embryonic stem cells
First Claim
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3. The cell population of either preceding claim, in which at least 5% of the cells express at least two of the following markers:
- Pdx1, Ngn3, insulin, IAPP, and Nkx6.1.
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Abstract
This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells. High quality populations of islet cells can be produced in commercial quantities for use in research, drug screening, or regenerative medicine.
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18 Claims
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3. The cell population of either preceding claim, in which at least 5% of the cells express at least two of the following markers:
- Pdx1, Ngn3, insulin, IAPP, and Nkx6.1.
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10-1. The method of claim 11, wherein the pPS cells are differentiated to form embryoid bodies, or cells with characteristics of hepatocytes or gut endothelium.
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11-2. The method of claim 11, wherein the mixture of islet cell differentiation factors comprises at least one of the following:
- activin A, nicotinamide, cyclopamine, betacellulin, and IGF-1.
Specification