Methods for the regeneration of pancreatic islets and expansion of pancreatic endocrine cells

• US 20030138949A1
• Filed: 12/10/2002
• Published: 07/24/2003
• Est. Priority Date: 12/12/2001
• Status: Abandoned Application

• Current Assignee: Childrens Hospital Los Angeles
• Original Assignee: Childrens Hospital Los Angeles
• Inventors: Bhushan, Anil, Warburton, David
• Application Number:
  US10/315,703
• Publication Number:
  US 20030138949A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: A61K 38/1825 Fibroblast growth factor [FGF]
• CPC Class Codes: C12N 2501/117 Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
• CPC Class Codes: C12N 2501/119 Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
• CPC Class Codes: C12N 5/0678 Stem cells; Progenitor cells; Precursor cells

Abstract

A method of facilitating the proliferation of pancreatic progenitor cells, particularly mammalian cells, is described. The method is carried out by administering an FGF polypeptide such as FGF-10 polypeptide to the cells in an amount effective to facilitate the proliferation thereof.

Claims

1. A method of facilitating the proliferation of pancreatic progenitor cells, comprising administering a FGF polypeptide to said cells in an amount effective to facilitate the proliferation thereof.

2. The method according to claim 1, wherein said administering step is carried out in vitro.

3. The method according to claim 1, wherein said pancreatic progenitor cells are mammalian pancreatic progenitor cells.

4. The method according to claim 1, wherein said pancreatic progenitor cells are human pancreatic progenitor cells.

5. The method according to claim 1, wherein said FGF polypeptide is selected from the group consisting of FGF-2, FGF-7, and FGF-10 polypeptides.

6. The method according to claim 1, wherein said FGF polypeptide is an FGF-10 polypeptide.

7. A method of facilitating the proliferation of mammalian pancreatic progenitor cells in vitro, comprising administering an FGF polypeptide to said cells in vitro in an amount effective to facilitate the proliferation thereof.

8. The method according to claim 7, wherein said pancreatic progenitor cells are human pancreatic progenitor cells.

9. The method according to claim 7, wherein said FGF polypeptide is selected from the group consisting of FGF-2, FGF-7, and FGF-10 polypeptides.

10. The method according to claim 7, wherein said FGF polypeptide is an FGF-10 polypeptide.

11. The method according to claim 7, wherein said FGF polypeptide is administered in an amount ranging from about 0.5 ng/ml to about 1000 ng/ml.

12. The method according to claim 7, wherein said FGF polypeptide is administered in an amount ranging from about 1 ng/ml to about 500 ng/ml.

13. Isolated mammalian pancreatic progenitor cells produced by the process of claim 7.