Microdevices for screening biomolecules
First Claim
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1. A device for analyzing components of a fluid sample, having a plurality of noncontiguous reactive sites, each of said sites comprising:
- (a) a substrate;
(b) an organic thinfilm chemisorbed or physisorbed on a portion of the surface of said substrate; and
(c) a biological moiety immobilized on said organic thinfilm;
wherein each of said sites may independently react with a component of the fluid sample and are separated from each other by a region of said substrate that is free of said organic thinfilm.
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Abstract
Methods and devices for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules immobilized on the surface of the devices of the present invention include proteins, polypeptides, polynucleotides, polysaccharides, phospolipids, and related unnatural polymers of biological relevance. These devices are useful drug development, functional proteomics and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.
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Citations
46 Claims
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1. A device for analyzing components of a fluid sample, having a plurality of noncontiguous reactive sites, each of said sites comprising:
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(a) a substrate;
(b) an organic thinfilm chemisorbed or physisorbed on a portion of the surface of said substrate; and
(c) a biological moiety immobilized on said organic thinfilm;
wherein each of said sites may independently react with a component of the fluid sample and are separated from each other by a region of said substrate that is free of said organic thinfilm. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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45. A device for analyzing components of a fluid sample, comprising:
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(a) a substrate;
(b) a plurality of parallel microchannels formed onto said substrate by a sealing gasket;
(c) a region for immobilizing at least one biological moiety within at least one of said parallel microchannels such that said biological moiety, once immobilized, may interact with a component of the fluid sample;
(d) a cover; and
,(e) one or more ports in fluidic communication with said channels for introducing or removing fluid from said channels. - View Dependent Claims (46)
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Specification