Device for chemical and biochemical reactions using photo-generated reagents

• US 20030143131A1
• Filed: 05/29/2002
• Published: 07/31/2003
• Est. Priority Date: 02/11/1998
• Status: Active Grant

• Current Assignee: University of Houston Law Center (University of Houston System), Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Gao, Xiaolian, Zhou, Xiaochuan, Gulari, Erdogan
• Granted Patent: US 7,838,466 B2
• Time in Patent Office: Days
• US Class Current: 422/186
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00434 Liquid crystal masks
• CPC Class Codes: B01J 2219/00439 using micromirror arrays
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00689 : using computers
• CPC Class Codes: B01J 2219/00711 : Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B01J 2219/00731 : Saccharides
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07B 2200/11 : Compounds covalently bound to a solid support
• CPC Class Codes: C07H 21/00 : Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07K 1/045 : using devices to improve synthesis, e.g. reactors, special vessels
• CPC Class Codes: C07K 1/047 : Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 40/12 : Libraries containing saccharides or polysaccharides, or derivatives thereof
• CPC Class Codes: C40B 50/14 : Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G03F 7/00 : Photomechanical, e.g. photolithographic, production of textured or patterned surfaces, e.g. printing surfaces; Materials therefor, e.g. comprising photoresists; Apparatus specially adapted therefor using photoresist structures for special production processes, see the relevant places, e.g. B44C, H01L, e.g. H01L21/00, H05K

Abstract

This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. Specifically, the method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.

Claims

1. A device for selectively converting photogenerated reagent precursors to photogenerated reagents comprising:

a) a substrate comprising at least one solid surface containing a plurality of spatially separated reaction sites; and

b) an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, wherein said optical system selectively irradiates a plurality of reaction sites.

2. The device of claim 1, wherein said spatial optical modulator is selected from the group consisting of a digital micromirror device, reflective liquid crystal display device, and transmissive liquid crystal display device.

3. The device of claim 2, wherein said digital micromirror device comprises a plurality of micromirrors.

4. The device of claim 3, wherein said micromirrors are individual rocking mirrors.

5. The device of claim 1, further comprising at least one filter in-line with said light source, wherein said filter is configured to generate a desired wavelength.

6. The device of claim 1, wherein said substrate is transparent.

7. The device of claim 1, further comprising a reagent manifold having one or more inlets and one or more outlets, said manifold configured to direct at least one reagent solution to said reaction site.

8. The device of claim 1, wherein said reaction sites are isolated reaction-wells.

9. The device of claim 1, further comprising a mechanism for controlling light intensity thereby controlling the generation of photogenerated reagents.

10. A device for selectively converting photogenerated reagent precursors to photogenerated reagents for effecting a chemical reaction comprising:

a) a substrate comprising at least one solid surface containing a plurality of spatially separated reaction sites, wherein said reaction sites comprise one or more reagents and contain a solution of at least one photogenerated reagent precursor; and

b) an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, which optical system selectively irradiates a plurality of reaction sites thereby converting the photogenerated reagent precursors to photogenerated reagents thereby effecting a chemical reaction.

11. The device of claim 10, wherein said spatial optical modulator is selected from the group consisting of digital micromirror device, reflective liquid crystal display device, and transmissive liquid crystal display device.

12. The device of claim 11, wherein said digital micromirror device comprises a plurality of micromirrors.

13. The device of claim 12, wherein said micromirrors are individual rocking mirrors.

14. The device of claim 10, further comprising at least one filter in-line with said light source, wherein said filter is configured to generate a desired wavelength.

15. The device of claim 10, wherein said substrate is transparent.

16. The device of claim 10, further comprising a reagent manifold having one or more inlets and one or more outlets, said manifold configured to direct at least one reagent solution to said reaction site.

17. The device of claim 10, wherein said reaction sites are isolated reaction-wells.

18. The device of claim 10, further comprising a mechanism for controlling light intensity thereby controlling the generation of photogenerated reagents.

19. A device for selectively converting photogenerated reagent precursors to photogenerated reagents for effecting deprotection of one or more initiating moieties comprising:

a) a substrate comprising at least one solid surface containing a plurality of spatially separated reaction sites, wherein said reaction sites comprise one or more initiating moieties and contain a solution of at least one photogenerated reagent precursor; and

b) an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, wherein said optical system selectively irradiates a plurality of reaction sites whereby said initiating moieties are deprotected by conversion of said photogenerated reagent precursors to photogenerated reagents.

20. The device of claim 19, wherein said spatial optical modulator is selected from the group consisting of digital micromirror device, reflective liquid crystal display device, and transmissive liquid crystal display device.

21. The device of claim 20, wherein said digital micromirror device comprises a plurality of micromirrors.

22. The device of claim 21, wherein said micromirrors are individual rocking mirrors.

23. The device of claim 19, further comprising at least one filter in-line with said light source, wherein said filter is configured to generate a desired wavelength.

24. The device of claim 19, wherein said substrate is transparent.

25. The device of claim 19, further comprising a reagent manifold having one or more inlets and one or more outlets, said manifold configured to direct at least one reagent solution to said reaction site.

26. The device of claim 19, wherein said reaction sites are isolated reaction-wells.

27. The device of claim 19, further comprising a mechanism for controlling light intensity thereby controlling the generation of photogenerated reagents.